Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments.

Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments. Research Abstract Details 

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Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments. Abstract Text:

Liang Li,James Q Boedicker,Rustem F Ismagilov,

In this paper we describe a multijunction microfluidic device for the injection of a substrate into an array of preformed plugs carried by an immiscible fluid in a microchannel. The device uses multiple junctions to inject substrate into preformed plugs without synchronization of the flow of substrate and the array of preformed plugs of reagent, which reduces cross-contamination of the plugs, eliminates formation of small droplets of substrate, and allows a greater range of injection ratios compared to that of a single T-junction. The device was based on a previously developed physical model for transport that was here adapted to describe injection and experimentally verified. After characterization, the device was applied to two biochemical assays, including evaluation of the enzymatic activity of thrombin and determination of the coagulation time of human blood plasma, which both provided reliable results. The reduction of cross-contamination and greater range of injection ratios achieved by this device may improve the processes that involve addition and titration of reagents into plugs, such as high-throughput screening of protein crystallization conditions.

Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments. Publishing Authors By Initials

L Li,JQ Boedicker,RF Ismagilov,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 2756-61

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 6

MONTH: 03

YEAR: 2007

Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments. Information

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LANGUAGE: eng

NlmUniqueID: 370536

Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: analysis

Chemical & Substance for Abstract: Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments. Information

Substance Name: Thrombin

Registry Number: EC 3.4.21.5

Grant and Affiliation Information for Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments.

AFFILIATION: Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM075827-02

ACRONYM: GM

MEDLINETA: Anal Chem

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