Use of monolithic supports in proteomics technology.

Use of monolithic supports in proteomics technology. Research Abstract Details 

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Use of monolithic supports in proteomics technology. Abstract Text:

Djuro Josic,James G Clifton,

An overview on the utilization of monoliths in proteomics technology will be given. Both silica- and polymer-based monoliths have broad use for microseparation of tryptic peptides in reversed-phase (RP) mode before identification by mass spectrometry (MS) or by MS/MS. For two-dimensional (2D) LC separation of peptides before MS or MS/MS analysis, a combination of ion-exchange, usually cation-exchange (CEX) chromatography with RP chromatography on monolithic supports can be employed. Immobilized metal ion affinity chromatography monoliths with immobilized Fe3+-ions are used for the isolation of phosphopeptides. Monoliths with immobilized affinity ligands are usually applied to the rapid separation of proteins and peptides. Miniaturized reactors with immobilized proteolytic enzymes are utilized for rapid on- or offline digestion of isolated proteins or protein mixtures prior to identification by LC-MS/MS. Monoliths also have broad potential for application in sample preparation, prior to further proteomic analyses. Monolithic supports with large pore sizes can be exploited for the isolation of nanoparticles, such as cells, organelles, viruses and protein aggregates. The potential for further adoption of monolithic supports in protein separation and enrichment of low abundance proteins prior to proteolytic digestion and final LC-MS/MS protein identification will be discussed.

Use of monolithic supports in proteomics technology. Publishing Authors By Initials

D Josic,JG Clifton,

For similar investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research abstracts see: investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research

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Use of monolithic supports in proteomics technology. Journal Published:

PUBLICATION TYPE: Review

Journal: Journal of chromatography. A

VOLUME: 1144

Page Numbers: 2-13

Journal Abbreviation:

ISSN: 0021-9673

DAY: 15

MONTH: 12

YEAR: 2006

Use of monolithic supports in proteomics technology. Information

Number of References: 122

LANGUAGE: eng

NlmUniqueID: 9318488

Use of monolithic supports in proteomics technology. Keywords Mesh Terms:

KEYWORDS: Tandem Mass Spectrometry

MESH TERMS: methods

Chemical & Substance for Abstract: Use of monolithic supports in proteomics technology. Information

Substance Name: Silicon Dioxide

Registry Number: 7631-86-9

Grant and Affiliation Information for Use of monolithic supports in proteomics technology.

AFFILIATION: Proteomics Core, COBRE Center for Cancer Research Development, Rhode Island Hospital, CORO West, One Hoppin St., Providence, RI 02903, USA. Djuro_Josic@brown.edu

Country: Netherlands

AGENCY: United States NCRR

GRANT: P20RR017695

ACRONYM: RR

MEDLINETA: J Chromatogr A

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