Use of DNAzymes for site-specific analysis of ribonucleotide modifications.

Use of DNAzymes for site-specific analysis of ribonucleotide modifications. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Use of DNAzymes for site-specific analysis of ribonucleotide modifications. Abstract Text:

Martin Hengesbach,Madeleine Meusburger,Frank Lyko,Mark Helm,Martin Hengesbach,Madeleine Meusburger,Frank Lyko,Mark Helm,Martin Hengesbach,Madeleine Meusburger,Frank Lyko,Mark Helm,Martin Hengesbach,Madeleine Meusburger,Frank Lyko,Mark Helm,

Post-transcriptional ribonucleotide modifications are widespread and abundant processes that have not been analyzed adequately due to the lack of appropriate detection methods. Here, two methods for the analysis of modified nucleotides in RNA are presented that are based on the quantitative and site-specific DNAzyme-mediated cleavage of the target RNA at or near the site of modification. Quantitative RNA cleavage is achieved by cycling the DNAzyme and its RNA substrate through repeated periods of heating and cooling. In a first approach, DNAzyme-directed cleavage directly 5' of the residue in question allows radioactive labeling of the newly freed 5'-OH. After complete enzymatic hydrolysis, the modification status can be assessed by two-dimensional thin layer chromatography. In a second approach, oligoribonucleotide fragments comprising the modification site are excised from the full-length RNA in an endonucleolytic fashion, using a tandem DNAzyme. The excised fragment is isolated by electrophoresis and submitted to further conventional analysis. These results establish DNAzymes as valuable tools for the site-specific and highly sensitive detection of ribonucleotide modifications.

Use of DNAzymes for site-specific analysis of ribonucleotide modifications. Publishing Authors By Initials

M Hengesbach,M Meusburger,F Lyko,M Helm,M Hengesbach,M Meusburger,F Lyko,M Helm,M Hengesbach,M Meusburger,F Lyko,M Helm,M Hengesbach,M Meusburger,F Lyko,M Helm,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Use of DNAzymes for site-specific analysis of ribonucleotide modifications. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: RNA (New York, N.Y.)

VOLUME: 14

Page Numbers: 180-7

Journal Abbreviation: RNA

ISSN: 1469-9001

DAY: 12

MONTH: 11

YEAR: 2007

Use of DNAzymes for site-specific analysis of ribonucleotide modifications. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9509184

Use of DNAzymes for site-specific analysis of ribonucleotide modifications. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Use of DNAzymes for site-specific analysis of ribonucleotide modifications. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Use of DNAzymes for site-specific analysis of ribonucleotide modifications.

AFFILIATION:

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: RNA

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Use of DNAzymes for site-specific analysis of ribonucleotide modifications Related Publications

• A methyl group controls conformational equilibrium in human mitochondrial tRNA(Lys).
• Adrenergic and peptidergic neuromuscular mechanisms in the human Fallopian tube, with special regard to cyclic influences.
• Bone graft substitutes for use in spinal fusions.
• Bone morphogenetic protein gene therapy for the induction of spinal arthrodesis.
• CT and radionuclide study of BMP-2 gene therapy-induced bone formation.
• Classic and atypical Spitz nevi: review of the literature.
• Collagenous spherulosis in an oral mucous cyst.
• Correlation between production amounts of DEHP and daily intake.
• Generation of canine-human Fc IgE chimeric antibodies for the determination of the canine IgE domain of interaction with FcvarepsilonRIalpha.
• Histopathologic findings and diagnostic difficulties posed with use of escharotic agents for treatment of skin lesions: a case report and review of the literature.
• Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin.
• In vivo endochondral bone formation using a bone morphogenetic protein 2 adenoviral vector.
• Local immunomodulation with CD4 and CD8 antibodies, but not cyclosporine A, improves osteogenesis induced by ADhBMP9 gene therapy.
• Measurement of long-ranged steric forces between polyelectrolyte layers physisorbed from 1 M NaCl.
• Multiple hyperkeratotic nodules on the arms.
• Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat.
• Percutaneous spinal fusion using bone morphogenetic protein-2 gene therapy.
• Problems in public health planning for orthodontic care.
• RNA silencing movement in plants.
• Remote reflections.
• Retraction of an intraperitoneal chemotherapy port: a case report and literature review.
• Surveillance of siRNA integrity by FRET imaging.
• The results of ankle arthrodesis.
• The role of the X-ray technician in cerebral angiography.
• Transmission features of frequency-selective components in the far infrared determined by terahertz time-domain spectroscopy.
• Use of DNAzymes for site-specific analysis of ribonucleotide modifications.
• [An improved lactose-peptone medium for the cultivation of chlorine-damaged E. coli bacteria]
• [Culturing of chlorine-damaged E. coli bacteria using the membrane filteration technic]
• [Preparation and properties of chlorination-damaged P. aeruginosa suspensions]
• [Suitability of thermal selection for the primary culture of Pseudomonas aeruginosa]