Use of chromosome substitution strains to identify seizure susceptibility loci in mice.

Use of chromosome substitution strains to identify seizure susceptibility loci in mice. Research Abstract Details 

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Use of chromosome substitution strains to identify seizure susceptibility loci in mice. Abstract Text:

Melodie R Winawer,Rachel Kuperman,Martin Niethammer,Steven Sherman,Daniel Rabinowitz,Irene Plana Guell,Christine A Ponder,Abraham A Palmer,

Seizure susceptibility varies among inbred mouse strains. Chromosome substitution strains (CSS), in which a single chromosome from one inbred strain (donor) has been transferred onto a second strain (host) by repeated backcrossing, may be used to identify quantitative trait loci (QTLs) that contribute to seizure susceptibility. QTLs for susceptibility to pilocarpine-induced seizures, a model of temporal lobe epilepsy, have not been reported, and CSS have not previously been used to localize seizure susceptibility genes. We report QTLs identified using a B6 (host) x A/J (donor) CSS panel to localize genes involved in susceptibility to pilocarpine-induced seizures. Three hundred fifty-five adult male CSS mice, 58 B6, and 39 A/J were tested for susceptibility to pilocarpine-induced seizures. Highest stage reached and latency to each stage were recorded for all mice. B6 mice were resistant to seizures and slower to reach stages compared to A/J mice. The CSS for Chromosomes 10 and 18 progressed to the most severe stages, diverging dramatically from the B6 phenotype. Latencies to stages were also significantly shorter for CSS10 and CSS18 mice. CSS mapping suggests seizure susceptibility loci on mouse Chromosomes 10 and 18. This approach provides a framework for identifying potentially novel homologous candidate genes for human temporal lobe epilepsy.

Use of chromosome substitution strains to identify seizure susceptibility loci in mice. Publishing Authors By Initials

MR Winawer,R Kuperman,M Niethammer,S Sherman,D Rabinowitz,IP Guell,CA Ponder,AA Palmer,

For similar biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research abstracts see: biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research

PUBMED ID PMID:

MEDLINE DATE:

Use of chromosome substitution strains to identify seizure susceptibility loci in mice. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Mammalian genome : official journal of the Interna

VOLUME: 18

Page Numbers: 23-31

Journal Abbreviation: Mamm. Genome

ISSN: 0938-8990

DAY: 22

MONTH: 01

YEAR: 2007

Use of chromosome substitution strains to identify seizure susceptibility loci in mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9100916

Use of chromosome substitution strains to identify seizure susceptibility loci in mice. Keywords Mesh Terms:

KEYWORDS: Species Specificity

MESH TERMS: genetics

Chemical & Substance for Abstract: Use of chromosome substitution strains to identify seizure susceptibility loci in mice. Information

Substance Name: Pilocarpine

Registry Number: 92-13-7

Grant and Affiliation Information for Use of chromosome substitution strains to identify seizure susceptibility loci in mice.

AFFILIATION: Department of Neurology, Columbia University, New York, NY 10032, USA. mw211@columbia.edu

Country: United States

AGENCY: United States NIMH

GRANT: MH70933

ACRONYM: MH

MEDLINETA: Mamm Genome

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