Use of a whole-cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants.

Use of a whole-cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants. Research Abstract Details 

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Use of a whole-cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants. Abstract Text:

Angela Keane,Peter C K Lau,Subhasis Ghoshal,Angela Keane,Peter C K Lau,Subhasis Ghoshal,Angela Keane,Peter C K Lau,Subhasis Ghoshal,

The whole-cell bioluminescent biosensor Pseudomonas putida F1G4 (PpF1G4), which contains a chromosomally-based sep-lux transcriptional fusion, was used as a tool for direct measurement of the bioavailability of hydrophobic organic compounds (HOCs) partitioned into surfactant micelles. The increased bioluminescent response of PpF1G4 in micellar solutions (up to 10 times the critical micellar concentration) of Triton X-100 and Brij 35 indicated higher intracellular concentrations of the test compounds, toluene, naphthalene, and phenanthrene, compared to control systems with no surfactants present. In contrast, Brij 30 caused a decrease in the bioluminescent response to the test compounds in single-solute systems, without adversely affecting cell growth. The decrease in bioluminescent response in the presence of Brij 30 did not occur in the presence of multiple HOCs extracted into the surfactant solutions from crude oil and creosote. The effect of the micellar solutions on the toluene biodegradation rate was consistent with the bioluminescent response in single-solute systems. None of the surfactants were toxic to PpF1G4 at the doses employed in this study, and PpF1G4 did not produce a bioluminescent response to the surfactants nor utilize them as growth substrates. TEM images suggest that the surfactants did not rupture the cell membranes. The results demonstrate that for Pseudomonas putida F1, nonionic surfactants such as Triton X-100 and Brij 35, at doses between 2 and 10 CMC, may increase the bioavailability and direct uptake of micellar phase HOCs that are common pollutants at contaminated sites.

Use of a whole-cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants. Publishing Authors By Initials

A Keane,PC Lau,S Ghoshal,A Keane,PC Lau,S Ghoshal,A Keane,PC Lau,S Ghoshal,

For similar chemical actions and uses: specialty uses of chemicals: surface-active agents research abstracts see: chemical actions and uses: specialty uses of chemicals: surface-active agents research

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Use of a whole-cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biotechnology and bioengineering

VOLUME: 99

Page Numbers: 86-98

Journal Abbreviation: Biotechnol. Bioeng.

ISSN: 1097-0290

DAY: 1

MONTH: Jan

YEAR: 2008

Use of a whole-cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants. Information

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LANGUAGE: eng

NlmUniqueID: 7502021

Use of a whole-cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants. Keywords Mesh Terms:

KEYWORDS: Surface-Active Agents

MESH TERMS: chemistry

Chemical & Substance for Abstract: Use of a whole-cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants. Information

Substance Name: Surface-Active Agents

Registry Number: 0

Grant and Affiliation Information for Use of a whole-cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants.

AFFILIATION: Department of Civil Engineering, McGill University, Macdonald Engineering Bldg, 817 Sherbrooke Street West, Montreal, Quebec, Canada.

Country: United States

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MEDLINETA: Biotechnol Bioeng

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