Use of a chimeric adenovirus vector enhances BMP2 production and bone formation.

Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Abstract Text:

Elizabeth A Olmsted-Davis,Zbigniew Gugala,Francis H Gannon,Patricia Yotnda,Robert E McAlhany,Ronald W Lindsey,Alan R Davis,

Recombinant adenoviral vectors have potential for the treatment of a variety of musculoskeletal defects and such gene therapy systems have been a recent research focus in orthopedic surgery. In studies reported here, two different adenovirus vectors have been compared for their ability to transduce human bone marrow mesenchymal stem cells (hBM-MSCs) and elicit bone formation in vivo. Vectors consisted either of standard adenovirus type 5 (Ad5) vector or a chimeric adenovirus type 5 vector that contains an adenovirus type 35 fiber (Ad5F35), which has been recently demonstrated to bestow a different cellular tropism, and a complete cDNA encoding human bone morphogenetic 2 (BMP2). Studies were also conducted to compare the transduction efficiency of these vectors using enhanced green fluorescent protein (GFP). hBM-MSCs transduced with Ad5F35 vectors had higher levels of transgene expression than those transduced with Ad5 vectors. The results also demonstrate that hBM-MSCs lack the coxsackie-adenovirus receptor (CAR), which is responsible for cellular adsorption of Ad5. Therefore, the data suggest that Ad5 virus adsorption to hBM-MSCs is inefficient. Ad5BMP2- or Ad5F35BMP2-transduced hBM-MSCs were also compared in an in vivo heterotopic bone formation assay. Mineralized bone was radiologically identified only in muscle that received the Ad5F35BMP2 transduced hBM-MSCs. In summary, Ad5F35BMP2 can efficiently transduce hBM-MSCs leading to enhanced bone formation in vivo.

Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Publishing Authors By Initials

EA Olmsted-Davis,Z Gugala,FH Gannon,P Yotnda,RE McAlhany,RW Lindsey,AR Davis,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

PUBMED ID PMID:

MEDLINE DATE:

Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Human gene therapy

VOLUME: 13

Page Numbers: 1337-47

Journal Abbreviation: Hum. Gene Ther.

ISSN: 1043-0342

DAY: 20

MONTH: Jul

YEAR: 2002

Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9008950

Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: metabolism

Chemical & Substance for Abstract: Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Use of a chimeric adenovirus vector enhances BMP2 production and bone formation.

AFFILIATION: Center for Cell and Gene Therapy, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA.

Country: United States

AGENCY: United States NIAMS

GRANT: R21AR484-01

ACRONYM: AR

MEDLINETA: Hum Gene Ther

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Use of a chimeric adenovirus vector enhances BMP2 production and bone formation Related Publications

• A new prognostic index in surgery and parenteral feeding: the ratio of triiodothyronine to reverse triiodothyronine in serum (T(3)/rT(3) ratio).
• A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction.
• Adenovirus-mediated BMP2 expression in human bone marrow stromal cells.
• Baxter allegiance prize acceptance.
• Cerebral metastases in malignant mesothelioma: case report and literature review.
• Childhood nephrosis.
• Comparison of the gross motor function measure and paediatric evaluation of disability inventory in assessing motor function in children undergoing selective dorsal rhizotomy.
• Coordinate transcriptional repression of liver fatty acid-binding protein and microsomal triglyceride transfer protein blocks hepatic very low density lipoprotein secretion without hepatosteatosis.
• Coordinating structural and functional synapse development: postsynaptic p21-activated kinase independently specifies glutamate receptor abundance and postsynaptic morphology.
• Cytokines, infections, stress, and dysphoric moods in breastfeeders and formula feeders.
• Development of pig blastocysts in vitro is altered by serum, bovine serum albumin and amino acids and vitamins.
• Drinking in the year after treatment as a predictor of three-year drinking outcomes.
• Emotion, motivation, and the brain: reflex foundations in animal and human research.
• Evidence for novel pH-dependent regulation of Candida albicans Rim101, a direct transcriptional repressor of the cell wall beta-glycosidase Phr2.
• Fc receptor homolog 3 is a novel immunoregulatory marker of marginal zone and B1 B cells.
• Female gamete preservation.
• Is Doppler ultrasound sufficient as the sole investigation before carotid endarterectomy?
• Lack of access to good healthcare & obese African-American women.
• Long-term outcomes in chronically hospitalized geriatric patients with schizophrenia: retrospective comparison of first generation and second generation antipsychotics.
• Measurement of the adhesion force between carbon nanotubes and a silicon dioxide substrate.
• Mechanisms underlying the rapid induction and sustained expression of synaptic homeostasis.
• Molecular inversion probe analysis of gene copy alterations reveals distinct categories of colorectal carcinoma.
• Osteoinduction by ex vivo adenovirus-mediated BMP2 delivery is independent of cell type.
• Perceptual and acoustic evidence of speaker adaptation to a thin pseudopalate.
• Physiologic, bronchoscopic, and bronchoalveolar lavage fluid findings in young children with recurrent wheeze and cough.
• Roles for Drosophila mushroom body neurons in olfactory learning and memory.
• Simultaneous shear and pressure sensor array for assessing pressure and shear at foot/ground interface.
• The effect of gonadal hormones and gender on anxiety and emotional learning.
• Use of a chimeric adenovirus vector enhances BMP2 production and bone formation.
• Women's responses to sexual aggression: the effects of childhood trauma, alcohol, and prior relationship.