Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans.

Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans. Abstract Text:

Huiyong Yin,Ling Gao,Hsin-Hsiung Tai,Laine J Murphey,Ned A Porter,Jason D Morrow,

Prostaglandins (PGs) derived from the enzymatic oxidation of arachidonic acid by the cyclooxygenases (COXs) are potent lipid mediators involved in human physiology and pathophysiology. Structurally similar compounds, the isoprostanes (IsoPs), are generated from the free radical-catalyzed oxidation of arachidonic acid independent of COX. IsoPs exhibit significant bioactivity and play a role in the pathogenesis of diseases associated with oxidant injury. As one of the major PGs, prostaglandin F(2alpha) (PGF(2alpha)) is present in human urine in significant concentrations and is presumed to be derived from COX activity. We determined, however, that levels of putative PGF(2alpha) in urine cannot be suppressed by nonsteroidal anti-inflammatory agents, suggesting that it is generated via another mechanism(s). An important difference between COX-derived PGF(2alpha) and the IsoPs is that the former is an optically pure compound, whereas IsoPs are racemic. Utilizing a rodent model of oxidative stress, we now show that significant amounts of compounds identical in all respects to PGF(2alpha) and its enantiomer, ent-PGF(2alpha), are formed in equal amounts esterified in tissue phospholipids, suggesting that these compounds are derived via the IsoP pathway. Further, employing liquid chromatography/mass spectrometry, the vast majority of putative PGF(2alpha) in human urine is derived from the free radical-initiated peroxidation of arachidonate independent of COX and is composed of PGF(2alpha) and its enantiomer, although the latter compound is approximately 2-fold more abundant. Thus, quantification of urinary PGF(2alpha) actually reflects oxidative stress status as opposed to COX activity. Indeed, levels of this compound are elevated in urine from cigarette smokers and in humans with hypercholesterolemia, two conditions associated with oxidant stress. The elucidation that urinary PGF(2alpha) in humans is derived from the IsoP pathway has implications regarding PG formation and inhibition in vivo.

Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans. Publishing Authors By Initials

H Yin,L Gao,HH Tai,LJ Murphey,NA Porter,JD Morrow,

For similar behavior and behavior mechanisms: behavior: habits: smoking research abstracts see: behavior and behavior mechanisms: behavior: habits: smoking research

PUBMED ID PMID:

MEDLINE DATE:

Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 329-36

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 15

MONTH: 11

YEAR: 2006

Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans. Keywords Mesh Terms:

KEYWORDS: Smoking

MESH TERMS: metabolism

Chemical & Substance for Abstract: Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans. Information

Substance Name: Prostaglandin-Endoperoxide Synthases

Registry Number: EC 1.14.99.1

Grant and Affiliation Information for Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans.

AFFILIATION: Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University, Nashville, TN 37232, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR00095

ACRONYM: RR

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans Related Publications

• AT-rich repeats associated with chromosome 22q11.2 rearrangement disorders shape human genome architecture on Yq12.
• Analysis of the replication of HIV-1 forced to use tRNAMet(i) supports a link between primer selection, translation and encapsidation.
• Assessment of cancer-related fatigue: implications for clinical diagnosis and treatment.
• Associations between Health-related Physical Fitness and Physical Activity Participation in Elementary School Children in Hong Kong: 2234: Board #147 June 1 9:00 AM - 10:30 AM.
• Behavioral interventions in treating anticipatory nausea and vomiting.
• Calculous diseases in infants and children.
• Cancer-related fatigue: causes, consequences, and management.
• Cancer-related fatigue: the scale of the problem.
• Clinician-Patient Communication about Physical Activity in an Underserved Population: 762: May 31 4:00 PM 4:15 PM.
• Clozapine markedly elevates pregnenolone in rat hippocampus, cerebral cortex, and serum: candidate mechanism for superior efficacy?
• Dissection of Tbx1 and Fgf interactions in mouse models of 22q11DS suggests functional redundancy.
• Effects of vitamin E on oxidative stress and atherosclerosis in an obese hyperlipidemic mouse model.
• Expression of epithelial mucins Muc1, Muc2, and Muc3 in ductal carcinoma in situ of the breast.
• Four-week course of radiation for breast cancer using hypofractionated intensity modulated radiation therapy with an incorporated boost.
• Hypothalamic-pituitary-adrenal axis and ethanol modulation of deoxycorticosterone levels in cynomolgus monkeys.
• Impact of forced selection of tRNAs on HIV-1 replication and genome stability highlight preferences for selection of certain tRNAs.
• Importance of A-loop complementarity with tRNAHis anticodon for continued selection of tRNAHis as the HIV reverse transcription primer.
• Mutations in GJB2, GJB6, and mitochondrial DNA are rare in African American and Caribbean Hispanic individuals with hearing impairment.
• Mutations in the TPsiC loop of E. coli tRNALys,3 have varied effects on in trans complementation of HIV-1 replication.
• Neuropsychological function and employment status in a welfare-to-work sample.
• Nucleotides within the anticodon stem are important for optimal use of tRNA(Lys,3) as the primer for HIV-1 reverse transcription.
• Olanzapine and fluoxetine administration and coadministration increase rat hippocampal pregnenolone, allopregnanolone and peripheral deoxycorticosterone: implications for therapeutic actions.
• Patterns and correlates of patient referral to surgeons for treatment of breast cancer.
• Quantification of F2-isoprostanes as a biomarker of oxidative stress.
• Seminal selenium concentrations and spermatozoal abnormalities in beef bulls.
• Sequential degradation of alphaII and betaII spectrin by calpain in glutamate or maitotoxin-stimulated cells.
• Structure of the calmodulin alphaII-spectrin complex provides insight into the regulation of cell plasticity.
• The role of Haemophilus somnus in early embryonic death. I. The effect of the organism on embryos by day 8 postbreeding.
• Urinary prostaglandin F2alpha is generated from the isoprostane pathway and not the cyclooxygenase in humans.
• tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation.