Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits.

Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits. Research Abstract Details 

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Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits. Abstract Text:

Daiva Dailidiene,Shumin Tan,Keiji Ogura,Maojun Zhang,Amy H Lee,Konstantin Severinov,Douglas E Berg,

BACKGROUND: The beta and beta' subunits of RNA polymerase are fused in all Helicobacters, but separate in most other taxa. Prior studies had shown that this fusion is not essential for viability in culture or in vivo, but had not tested it for potentially important quantitative effects on phenotype. METHODS: The effect of separating rpoB and rpoC sequences on Helicobacter pylori growth was tested in culture and during mouse infection. RESULTS: Derivatives of strains X47 and SS1 carrying this "rpoBCsplit" allele colonized mice less vigorously than their wild-type parents in competition tests. With X47 rpoBCsplit, this reduced vigor was evident in wild-type mice, whereas with SS1 rpoBCsplit it was seen only in cytokine IL-10- and IL-12beta-deficient mice. In culture, the rpoBCsplit allele sensitized each of four strains tested (X47, SS1, 88-3887, and AM1) to urea, a metabolite that is secreted into the gastric mucosa; urea sensitization was more severe in X47 than in SS1 genetic backgrounds. The rpoBCsplit allele also caused poorer growth on Ham's F12 agar, a nutritionally limiting medium, but had little effect on sensitivity to mild acidity. CONCLUSIONS: H. pylori's normal RNA polymerase beta-beta' subunit fusion contributes quantitatively to fitness. We propose that urea, although important to H. pylori in vivo, also be considered inhibitory; and that H. pylori's natural beta-beta' subunit fusion helps it cope with urea exposure.

Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits. Publishing Authors By Initials

D Dailidiene,S Tan,K Ogura,M Zhang,AH Lee,K Severinov,DE Berg,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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MEDLINE DATE:

Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Helicobacter

VOLUME: 12

Page Numbers: 103-11

Journal Abbreviation: Helicobacter

ISSN: 1083-4389

DAY: 3

MONTH: Apr

YEAR: 2007

Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9605411

Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits. Information

Substance Name: RNA polymerase beta subunit

Registry Number: EC 2.7.7.6

Grant and Affiliation Information for Urea sensitization caused by separation of Helicobacter pylori RNA polymerase beta and beta' subunits.

AFFILIATION: Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R03 AI057826

ACRONYM: AI

MEDLINETA: Helicobacter

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