Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells.

Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells. Abstract Text:

Kenji Kuwayama,Hiroyuki Inoue,Tatsuyuki Kanamori,Kenji Tsujikawa,Hajime Miyaguchi,Yuko Iwata,Seiji Miyauchi,Naoki Kamo,Tohru Kishi,

3,4-Methylenedioxymethamphetamine (MDMA) is an illegal amphetamine-type stimulant (ATS) that is abused orally in the form of tablets for recreational purposes. The aim of this work is to investigate the absorption mechanism of MDMA and other related compounds that often occur together in ATS tablets, and to determine whether such tablet components interact with each other in intestinal absorption. The characteristics of MDMA uptake by the human intestinal epithelial Caco-2 cell line were investigated. The Michaelis constant and the maximal uptake velocity at pH 6.0 were 1.11 mM and 13.79 nmol/min/mg protein, respectively, and the transport was electroneutral. The initial uptake rate was regulated by both intra- and extracellular pH. MDMA permeation from the apical to the basolateral side was inferior to that in the reverse direction, and a decrease in apical pH enhanced MDMA permeation from the basolateral to the apical side. These facts indicate that this transport system may be an antiporter of H(+). However, under physiological conditions, the proton gradient cannot drive the MDMA uptake because it is inwardly directed. Large concentration differences of MDMA itself drive this antiporter. Various compounds with similar amine moieties inhibited the uptake, but substrates of organic cation transporters (OCT1-3) and an H(+)-coupled efflux antiporter, MATE, were not recognized.

Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells. Publishing Authors By Initials

K Kuwayama,H Inoue,T Kanamori,K Tsujikawa,H Miyaguchi,Y Iwata,S Miyauchi,N Kamo,T Kishi,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochimica et biophysica acta

VOLUME: 1778

Page Numbers: 42-50

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 8

MONTH: 09

YEAR: 2007

Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 217513

Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells.

AFFILIATION: National Research Institute of Police Science, 6-3-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan; Laboratory of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biochim Biophys Acta

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells Related Publications

• A Fatal Case of Suspected Anaphylaxis with Cefoperazone and Sulbactam: LC-MS Analysis.
• A simple but remarkably effective device for forming the C8-C14 polycyclic ring system of halichondrin B.
• Aggregation behavior of tetraenoic fatty acids in aqueous solution.
• Antitumor and immunosuppressive activities of lankacidin-group antibiotics: structure-activity relationships.
• Association between duration of daily visual display terminal work and insomnia among local government clerks in Japan.
• Axon or dendrite? cell biology and molecular pathways for neuronal cell asymmetry.
• Carp hepatopancreatic DNase I: biochemical, molecular, and immunological properties.
• Catalytic Ni/Cr-mediated macrocyclization without use of high-dilution techniques.
• Characterization of NADPH-dependent ubiquinone reductase activity in rat liver cytosol: effect of various factors on ubiquinone-reducing activity and discrimination from other quinone reductases.
• Complexation of fatty acids and fatty acid-CoAs with synthetic O-methylated polysaccharides.
• Deoxyribonuclease I from rat urine: affinity purification, characterization, and immunochemical studies.
• Fe/Cr- and Co/Cr-mediated catalytic asymmetric 2-haloallylations of aldehydes.
• H pylori seropositivity and cytokine gene polymorphisms.
• Human genetically polymorphic deoxyribonuclease: purification, characterization, and multiplicity of urine deoxyribonuclease I.
• Molecular, biochemical and immunological analyses of canine pancreatic DNase I.
• New detection method for ribonuclease 2 (RNase 2) using immunoblotting with specific antibody.
• New detection method for uropepsinogen (PGA) using isoelectric focusing and immunoblotting techniques.
• Polarization-Independent Optical Fiber Modulator by use of Polymer-Dispersed Liquid Crystals.
• Product-regulation mechanisms for fatty acid biosynthesis catalyzed by Mycobacterium smegmatis FAS I.
• Purification and characterization of genetically polymorphic deoxyribonuclease I from human kidney.
• Purification of a young age-related glycoprotein (Ugl-Y) from normal human urine.
• Spatial light modulators for projection displays.
• Three-dimensional large-screen display with reflection-mode spatial light modulators and a single-projection optical system: analysis of a retardation-modulation method.
• Total synthesis and stereochemistry of mycolactone f.
• Total synthesis and stereochemistry of pinnatoxins B and C.
• Unified total synthesis of pteriatoxins and their diastereomers.
• Uptake of 3,4-methylenedioxymethamphetamine and its related compounds by a proton-coupled transport system in Caco-2 cells.
• ["Sympathy" and "speech"--their significance in nursing practice]
• [Basic and clinical results with acetyl spiramycin in surgery]
• [Nursing students among the young generation--their thought structures and relationship with the instructors]