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Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals.

Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals. Research Abstract Details 

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  • Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals. Abstract Text:

    dev k chatterjeeDev K Chatterjee,abdul j rufaihahAbdul J Rufaihah,yong zhangYong Zhang,dev k chatterjeeDev K Chatterjee,abdul j rufaihahAbdul J Rufaihah,yong zhangYong Zhang,

    Upconversion fluorescence imaging technique with excitation in the near-infrared (NIR) region has been used for imaging of biological cells and tissues. This has several advantages, including absence of photo-damage to living organisms, very low auto-fluorescence, high detection sensitivity, and high light penetration depth in biological tissues. In this report we demonstrate the use of a new upconversion fluorophore, lanthanide doped nanocrystals, for imaging of cells and some deep tissues in animal. Polyethyleneimine (PEI) coated NaYF(4):Yb,Er nanoparticles were synthesized, which produce very strong upconversion fluorescence when excited at 980nm by a NIR laser. The nanoparticles were shown to be stable in physiologic buffered saline (PBS), non-toxic to bone marrow stem cells, and resistant to photo-bleaching. The nanoparticles delivered into some cell lines or injected intradermally and intramuscularly into some tissues either near the body surface or deep in the body of rats showed visible fluorescence, when exposed to a 980nm NIR laser. To the best of our knowledge, this represents the first demonstration of use of upconversion fluorophores for cellular and tissue imaging.

    Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals. Publishing Authors By Initials

    dk chatterjeeDK Chatterjee,aj rufaihahAJ Rufaihah,y zhangY Zhang,dk chatterjeeDK Chatterjee,aj rufaihahAJ Rufaihah,y zhangY Zhang,

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    Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Biomaterials

    VOLUME: 29

    Page Numbers: 937-43

    Journal Abbreviation: Biomaterials

    ISSN: 0142-9612

    DAY: 3

    MONTH: 12

    YEAR: 2007

    Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals. Information

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    LANGUAGE: eng

    NlmUniqueID: 8100316

    Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals. Keywords Mesh Terms:

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    Grant and Affiliation Information for Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals.

    AFFILIATION: Division of Bioengineering, National University of Singapore, Singapore 117574, Singapore.

    Country: England

    England Research PublicationEngland Research Publication

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    MEDLINETA: Biomaterials

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