Up-regulation of p55 TNF alpha-receptor in dorsal root ganglia neurons following lumbar facet joint injury in rats.

Up-regulation of p55 TNF alpha-receptor in dorsal root ganglia neurons following lumbar facet joint injury in rats. Research Abstract Details 

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Up-regulation of p55 TNF alpha-receptor in dorsal root ganglia neurons following lumbar facet joint injury in rats. Abstract Text:

Yoshihiro Sakuma,Seiji Ohtori,Masayuki Miyagi,Tetsu Ishikawa,Gen Inoue,Hideo Doya,Takana Koshi,Toshinori Ito,Masaomi Yamashita,Kazuyo Yamauchi,Munetaka Suzuki,Hideshige Moriya,Kazuhisa Takahashi,

The rat L5/6 facet joint is multisegmentally innervated from the L1 to L6 dorsal root ganglia (DRG). Tumor necrosis factor (TNF) is a known mediator of inflammation. It has been reported that satellite cells are activated, produce TNF and surround DRG neurons innervating L5/6 facet joints after facet injury. In the current study, changes in TNF receptor (p55) expression in DRG neurons innervating the L5/6 facet joint following facet joint injury were investigated in rats using a retrograde neurotransport method followed by immunohistochemistry. Twenty rats were used for this study. Two crystals of Fluorogold (FG; neurotracer) were applied into the L5/6 facet joint. Seven days after surgery, the dorsal portion of the capsule was cut in the injured group (injured group n = 10). No injury was performed in the non-injured group (n = 10). Fourteen days after the first application of FG, bilateral DRGs from T13 to L6 levels were resected and sectioned. They were subsequently processed for p55 immunohistochemistry. The number of FG labeled neurons and number of FG labeled p55-immunoreactive (IR) neurons were counted. FG labeled DRG neurons innervating the L5/6 facet joint were distributed from ipsilateral L1 to L6 levels. Of FG labeled neurons, the ratio of DRG neurons immunoreactive for p55 in the injured group (50%) was significantly higher than that in the non-injured group (13%). The ratio of p55-IR neurons of FG labeled DRG neurons was significantly higher in total L1 and L2 DRGs than that in total L3, 4, 5 and 6 DRGs in the injured group (L1 and 2 DRG, 67%; L3, 4, 5 and 6 DRG, 37%, percentages of the total number of p55-IR neurons at L1 and L2 level or L3-6 level/the total number of FG-labeled neurons at L1 and L2 level or L3-6 level). These data suggest that up-regulation of p55 in DRG neurons may be involved in the sensory transmission from facet joint injury. Regulation of p55 in DRG neurons innervating the facet joint was different between upper DRG innervated via the paravertebral sympathetic trunks and lower DRG innervated via other direct routes.

Up-regulation of p55 TNF alpha-receptor in dorsal root ganglia neurons following lumbar facet joint injury in rats. Publishing Authors By Initials

Y Sakuma,S Ohtori,M Miyagi,T Ishikawa,G Inoue,H Doya,T Koshi,T Ito,M Yamashita,K Yamauchi,M Suzuki,H Moriya,K Takahashi,

For similar musculoskeletal system: skeleton: joints: zygapophyseal joint research abstracts see: musculoskeletal system: skeleton: joints: zygapophyseal joint research

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Up-regulation of p55 TNF alpha-receptor in dorsal root ganglia neurons following lumbar facet joint injury in rats. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: European spine journal : official publication of t

VOLUME: 16

Page Numbers: 1273-8

Journal Abbreviation:

ISSN: 0940-6719

DAY: 28

MONTH: 04

YEAR: 2007

Up-regulation of p55 TNF alpha-receptor in dorsal root ganglia neurons following lumbar facet joint injury in rats. Information

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LANGUAGE: eng

NlmUniqueID: 9301980

Up-regulation of p55 TNF alpha-receptor in dorsal root ganglia neurons following lumbar facet joint injury in rats. Keywords Mesh Terms:

KEYWORDS: Zygapophyseal Joint

MESH TERMS: metabolism

Chemical & Substance for Abstract: Up-regulation of p55 TNF alpha-receptor in dorsal root ganglia neurons following lumbar facet joint injury in rats. Information

Substance Name: recombinant human tumor necrosis factor-

Registry Number: 0

Grant and Affiliation Information for Up-regulation of p55 TNF alpha-receptor in dorsal root ganglia neurons following lumbar facet joint injury in rats.

AFFILIATION: Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba 260-8670, Japan.

Country: Germany

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MEDLINETA: Eur Spine J

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