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Unusual organization associated to a tandem of IS231 may yield two peculiar cloverleaf secondary structures.

Unusual organization associated to a tandem of IS231 may yield two peculiar cloverleaf secondary structures. Research Abstract Details 

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  • Unusual organization associated to a tandem of IS231 may yield two peculiar cloverleaf secondary structures. Abstract Text:

    dong xuDong Xu,jean-charles Jean-Charles ,dong xuDong Xu,jean-charles Jean-Charles ,

    A 5.7-kb EcoRI fragment was cloned from plasmid DNA of Bacillus thuringiensis strain M15. It contains two insertion sequences (IS), IS231M2 and -M1 in the 5'-3' order, arranged in tandem, in same orientation, separated by a 540-bp region. The primary structure is typical of a composite transposon, here of 3847 bp in length, for which the name Tn231M is proposed. Each IS is delimited by 18-bp inverted repeats (IR), and flanked by 11-bp direct repeats (DR). Both IS share 99.3% nucleotide identities. IS231M1 has a single open reading frame (ORF) which encodes a putative 477-amino-acid transposase. IS231M2 has two smaller ORFs: ORF1 and ORF2, which could code for polypeptides of 329 and 118 amino acids in length, respectively. Further analysis reveals that the regions upstream of IS231M2, and downstream of -M1, and the 540-bp region, contain additional pairs of IR and DR. Interestingly, potential annealing between all pairs of IR and DR could generate two unusual cloverleaf secondary structures.

    Unusual organization associated to a tandem of IS231 may yield two peculiar cloverleaf secondary structures. Publishing Authors By Initials

    d xuD Xu,jc JC ,d xuD Xu,jc JC ,

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    Unusual organization associated to a tandem of IS231 may yield two peculiar cloverleaf secondary structures. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: DNA sequence : the journal of DNA sequencing and m

    VOLUME: 18

    Page Numbers: 288-94

    Journal Abbreviation: DNA Seq.

    ISSN: 1042-5179

    DAY: 1

    MONTH: Aug

    YEAR: 2007

    Unusual organization associated to a tandem of IS231 may yield two peculiar cloverleaf secondary structures. Information

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    LANGUAGE: eng

    NlmUniqueID: 9107800

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    Grant and Affiliation Information for Unusual organization associated to a tandem of IS231 may yield two peculiar cloverleaf secondary structures.

    AFFILIATION: Agriculture and Agri-Food Canada, Research Centre, Québec, Canada.

    Country: England

    England Research PublicationEngland Research Publication

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    MEDLINETA: DNA Seq

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