Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes.

Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes. Abstract Text:

R G Evans,A Norman,

The nature, degree, and kinetics of unscheduled thymidine incorporation previously shown to occur in 90 % of irradiated lymphocytes was stud-incorporation was sever ely depressed i n t h e presence of 10(-4) M acriflavine and by low temperature, but was unaffected by 10(-3) M hydroxyurea or caffeine. Over a dose range of 25 to 400 ergs/mm2, the uptake of thymidine was increased by a factor of only 1.6, although the survival of lymphocytes, measured 5 days after irradiation, decreased by almost two orders of magnitude. (The survival curve suggests that 90% of the lymphocytes have a D0 of 35 ergs/mm(2) and 10 % have a D0 of 250 ergs/mm(2).) After exposure to 25 ergs/mm(2), over 70 % of the cells survived for 5 days in culture; moreover, cells which had been stimulated by this dose to incorporate thymidine transformed and divided after exposure to phytohema-glutinin. The final uptake of thymidine was significantly greater when a total dose of 75 ergs/mm(2) was fractionated into three doses of 25 ergs/mm(2) given at six hourly intervals than when it was given as a single dose. The degree of thymidine incorporation and the fraction of leukemic cells labeled were not significantly different from those in normal lymphocytes.

Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes. Publishing Authors By Initials

RG Evans,A Norman,

For similar environment and public health: environment: meteorological factors: atmosphere: weather: sunlight: ultraviolet rays research abstracts see: environment and public health: environment: meteorological factors: atmosphere: weather: sunlight: ultraviolet rays research

PUBMED ID PMID:

MEDLINE DATE:

Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Radiation research

VOLUME: 36

Page Numbers: 287-98

Journal Abbreviation: Radiat. Res.

ISSN: 0033-7587

DAY: 3

MONTH: Nov

YEAR: 1968

Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 401245

Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes. Keywords Mesh Terms:

KEYWORDS: Ultraviolet Rays

MESH TERMS: pharmacokinetics

Chemical & Substance for Abstract: Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes.

AFFILIATION: Biophysics Division, Sloan-Kettering Cancer Institute, New York, New York, USA.

Country: United States

AGENCY: United States CSAT

GRANT: 5-TI-RH81-02

ACRONYM: TI

MEDLINETA: Radiat Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes Related Publications

• A brief review of the history and use of the daresbury ;miyake' monochromator for surface science.
• Abdominal aortic aneurysm: pathogenesis and implications for management.
• Association between AMPD1 gene polymorphism and coagulation factors in patients with coronary heart disease.
• Association between osteopontin and human abdominal aortic aneurysm.
• Benzopyrans as selective estrogen receptor beta agonists (SERBAs). Part 3: synthesis of cyclopentanone and cyclohexanone intermediates for C-ring modification.
• Benzopyrans as selective estrogen receptor beta agonists (SERBAs). Part 5: Combined A- and C-ring structure-activity relationship studies.
• Conservative mutageneic perturbations of amino acids connecting helix 12 in the 1alpha,25(OH)2-D3 receptor (VDR) to the ligand cause significant transactivational effects.
• Design and synthesis of quinolin-2(1H)-one derivatives as potent CDK5 inhibitors.
• Dopamine dysregulation syndrome: implications for a dopamine hypothesis of bipolar disorder.
• Dry period length in US Jerseys: characterization and effects on performance.
• Effect of unitization on associative recognition in amnesia.
• Ghrelin and measures of satiety are altered in polycystic ovary syndrome but not differentially affected by diet composition.
• Homocysteine and abdominal aortic aneurysms.
• How basic is basic science?
• It all make sense: biomedical knowledge, causal connections and memory in the novice diagnostician.
• Minireview: vitamin D receptor: new assignments for an already busy receptor.
• New insights into Vitamin D sterol-VDR proteolysis, allostery, structure-function from the perspective of a conformational ensemble model.
• Physical Activity Intensity and Duration Patterns in Mild-to-Moderately Depressed Adults: 2434: Board #113 June 1 3:30 PM -5:00 PM.
• Preterm labour. Cervical function and prematurity.
• Quality management systems in ART: are they really needed? An Australian clinic's experience.
• Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments.
• Structure-activity relationship (SAR) investigations of substituted imidazole analogs as TRPV1 antagonists.
• Structure-activity relationships of 3,4-dihydro-1H-quinazolin-2-one derivatives as potential CDK5 inhibitors.
• Successful breastfeeding after discharge of preterm and sick newborn infants.
• Teaching session C: data collection and statistics in radiotherapy : size matters.
• The role of biomedical knowledge in diagnosis of difficult clinical cases.
• The use of anti-mullerian hormone in predicting menstrual response after weight loss in overweight women with polycystic ovary syndrome.
• Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes.
• Vs03 abdominal aortic aneurysm: insight into pathogenesis, biomarkers and targets for medical therapy.
• Within-herd heritability estimated with daughter-parent regression for yield and somatic cell score.