Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity.

Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity. Abstract Text:

The zone of polarizing activity is the primary signaling center controlling anterior-posterior patterning of the amniote limb bud. The autopodial interdigits (IDs) are secondary signaling centers proposed to determine digit identity by acting on the cells of the digital ray. Here, we focus on events accompanying digital fate determination and define a region of the digital ray that expresses Sox9 and Bmpr1b and is phosphorylated-SMAD1/5/8 (p-SMAD1/5/8) positive. We name this region the phalanx-forming region (PFR), and show that the PFR cells arise from the distal subridge mesenchyme of digital ray. This phalanx-forming cell lineage is subsequently committed to the cartilage lineage; the fate of these cells is initially labile but becomes fixed as they are incorporated into the condensed cartilage of the digit primordium. Using an in vivo reporter assay, we establish that each digital PFR has a unique p-SMAD1/5/8 activity signature. In addition, we show that changes in this activity correlate with the identity of the digit that forms after experimental manipulation, supporting the idea that threshold signaling levels can lead to different developmental outcomes in a morphogenetic field. Our data define the molecular profile of the PFR, and we propose a model for understanding formation and variation of digits during autopodial development.

Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 105

Page Numbers: 4185-90

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 1091-6490

DAY: 11

MONTH: 03

YEAR: 2008

Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity.

AFFILIATION: Department of Anatomy University of Wisconsin, 1300 University Avenue, Madison, WI 53706.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity Related Publications

• 'Off-label' drug use in sexual medicine treatment.
• A novel stimulus artifact removal technique for high-rate electrical stimulation.
• Ampullary cancer in chemical workers.
• Asphyxial games in children and adolescents.
• Decreased survival of mosquito cells after stable transfection with a Drosophila ecdysteroid response element: possible involvement of a 40 kDa DNA binding protein.
• Effects of brief periods of myocardial ischemia on regional myocardial function and creatine kinase release in conscious dogs and baboons.
• GABAergic neurons comprise a major cell type in rodent visual relay nuclei: an immunocytochemical study of pretectal and accessory optic nuclei.
• Genetic data and the listing of species under the u.s. Endangered species act.
• Histone H1-like, lysine-rich low complexity amino acid extensions in mosquito ribosomal proteins RpL23a and RpS6 have evolved independently.
• Indiana pouch stone formation caused by a long-forgotten metallic ball bearing.
• Large-scale dairy operations: assessing concerns of neighbors about quality-of-life issues.
• Life events; their role in onset and relapse in psychosis, research utilizing semi-structured interview methods: a literature review.
• Liver transplantation for hepatopulmonary syndrome (HPS): what is the MESSAGE?
• MR imaging of soft-tissue hemangiomas: correlation with pathologic findings.
• Management of cancer pain: basic principles and neuropathic cancer pain.
• Men who have sex with men: estimated population sizes and mortality rates by race/ethnicity, Miami-Dade County, Florida.
• Modulation of pulmonary endothelial endothelin B receptor expression and signaling: implications for experimental hepatopulmonary syndrome.
• Neurotensin and cholecystokinin coexistence within neurons of the ventral mesencephalon: projections to forebrain.
• Oxygen desaturation during sleep in hepatopulmonary syndrome.
• Pain management.
• Pathology of arterial lesions amenable to percutaneous transluminal angioplasty.
• Pentoxifylline attenuation of experimental hepatopulmonary syndrome.
• Renal venous thrombosis in the newborn.
• Screening for haematological and iron-related abnormalities in elite athletes-Analysis of 576 cases.
• Steam penetration into containers for surgical dressings.
• Sterilisation of radium needles and gynaecological applicators.
• The clinical utility of screening of biochemical parameters in elite athletes: analysis of 100 cases.
• The importance of good teamwork.
• Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity.
• Utility of pulse oximetry screening for hepatopulmonary syndrome.