Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton.

Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton. Abstract Text:

The spectrin cytoskeleton assembles within discrete regions of the plasma membrane in a wide range of animal cell types. Although recent studies carried out in vertebrate systems indicate that spectrin assembly occurs indirectly through the adapter protein ankyrin, recent studies in Drosophila have established that spectrin can also assemble through a direct ankyrin-independent mechanism. Here we tested specific regions of the spectrin molecule for a role in polarized assembly and function. First, we tested mutant beta-spectrins lacking ankyrin binding activity and/or the COOH-terminal pleckstrin homology (PH) domain for their assembly competence in midgut, salivary gland, and larval brain. Remarkably, three different assembly mechanisms operate in these three cell types: 1) neither site was required for assembly in salivary gland; 2) only the PH domain was required in midgut copper cells; and 3) either one of the two sites was sufficient for spectrin assembly in larval brain. Further characterization of the PH domain revealed that it binds strongly to lipid mixtures containing phosphatidylinositol 4,5-bisphosphate (PIP(2)) but not phosphatidylinositol 3,4,5-trisphosphate. A K8Q mutation in the lipid binding region of the PH domain eliminated the PIP(2) interaction in vitro, yet the mutant protein retained full biological function in vivo. Reporter gene studies revealed that PIP(2) and the spectrin PH domain codistribute with one another in cells but not with authentic wild type alphabeta-spectrin. Thus, it appears that the PH domain imparts membrane targeting activity through a second mechanism that takes precedence over its PIP(2) binding activity.

Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 283

Page Numbers: 12643-53

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 19

MONTH: 02

YEAR: 2008

Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton.

AFFILIATION: Laboratory for Molecular Biology and Department of Biological Sciences.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton Related Publications

• Antioxidative effect of Bacteroides thetaiotaomicron extracts: superoxide dismutase identification.
• Bone mass density, subsequent risk of colon cancer and survival in postmenopausal women.
• Cochlear implant: experience of the Lyon team.
• Copper cells and stomach acid secretion in the Drosophila midgut.
• Epidemiology of Toxocariasis in the Montreal area. Prevalence of Toxocara and other helminth ova in dogs and soil.
• Escherichia coli STb toxin and colibacillosis: knowing is half the battle.
• Escherichia coli STb toxin binding to sulfatide and its inhibition by carragenan.
• Functional links between membrane transport and the spectrin cytoskeleton.
• Gain-of-Function Mutations in the Extracellular Domain of KIT Are Common in Canine Mast Cell Tumors.
• Heterogeneity of Escherichia coli STb enterotoxin isolated from diseased pigs.
• Melioidotic osteomyelitis of the femur occurring in a traveler.
• Méningiomes de la face postérieure du rocher: aspects diagnostiques et therapeutiques.
• Population response to habitat fragmentation in a stream-dwelling brook trout population.
• Structure and evolution of the actin crosslinking proteins.
• Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton.
• [Autonomous tuberculosis of the subglottis]
• [Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)]
• [Marriage of very deaf persons]
• [Monitoring of antibiotic resistance of gram negative anaerobes.]
• [Not Available.]
• [Not Available.]
• [Post-traumatic cholesteatoma of the canal and middle ear]
• [Virus exretion and antigenic response after vaccination with attenuated (Sabin) type 1 poliomyelitis viruses.]