Understanding microchannel culture: parameters involved in soluble factor signaling.

Understanding microchannel culture: parameters involved in soluble factor signaling. Research Abstract Details 

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Understanding microchannel culture: parameters involved in soluble factor signaling. Abstract Text:

Hongmei Yu,Caroline M Alexander,David J Beebe,

While the importance of autocrine-paracrine signaling in vivo is clear, the ability to study the effects of secreted endogenous factors in vitro is hampered by canonical culture platforms. In multi-well plates, the large air-liquid interface gives rise to convective flows that continually mix the fluid disrupting the local diffusion-based accumulation. Simple microchannels provide a more controlled microenvironment that can be used to study secreted factor effects. Here, we utilize microchannel culture to examine basic culture parameters and their interactions using normal mammary gland epithelial cells (NMuMG). The following parameters were studied: (1) cell density (80 vs. 240 cells mm(-2)), (2) exogenous growth factors (epidermal growth factor [EGF] vs. fetal bovine serum), (3) medium change frequency (1 h, 4 h, 12 h), and (4) culture platform (microchannels vs. 96-well plates). The cells exhibited increased growth rates in microchannels as compared to 96-well plates. Cell proliferation increased as the frequency of media change decreased. For the microchannel geometries used, important threshold concentrations were reached in a few hours. In aggregate, the results indicate that the function of the four factors and their interactions on NMuMG growth are spatially and temporally related by molecular diffusion in the controlled microchannel space. The convective-free microchannel environment may prove useful for studying soluble factor signaling in vitro, and to test models and predictions of autocrine-paracrine signaling.

Understanding microchannel culture: parameters involved in soluble factor signaling. Publishing Authors By Initials

H Yu,CM Alexander,DJ Beebe,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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MEDLINE DATE:

Understanding microchannel culture: parameters involved in soluble factor signaling. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Lab on a chip

VOLUME: 7

Page Numbers: 726-30

Journal Abbreviation:

ISSN: 1473-0197

DAY: 19

MONTH: 04

YEAR: 2007

Understanding microchannel culture: parameters involved in soluble factor signaling. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101128948

Understanding microchannel culture: parameters involved in soluble factor signaling. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: physiology

Chemical & Substance for Abstract: Understanding microchannel culture: parameters involved in soluble factor signaling. Information

Substance Name: Epidermal Growth Factor

Registry Number: 62229-50-9

Grant and Affiliation Information for Understanding microchannel culture: parameters involved in soluble factor signaling.

AFFILIATION: Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA.

Country: England

AGENCY: United States NCI

GRANT: K25-CA104162-02

ACRONYM: CA

MEDLINETA: Lab Chip

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