Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor.

Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor. Abstract Text:

Kenneth Robzyk,Handy Oen,Grant Buchanan,Lisa M Butler,Wayne D Tilley,Atin K Mandal,Neal Rosen,Avrom J Caplan,

The mechanisms underlying androgen receptor (AR)-mediated progression of prostate cancer following androgen ablation have yet to be fully determined. On this basis we screened naturally occurring mutants of human AR for hormone-independent activity using a yeast model system. An initial screen of 43 different mutants revealed that ARs having a Leu701His mutation (AR(L701H)) exhibited hormone-independent activation of a lacZ reporter gene. The AR(L701H) mutant bound dihydrotestosterone to a similar extent as did wild type AR, although its ability to be induced by hormone for transactivation was reduced substantially. Subsequent studies focused on the dependence of AR(L701H) on molecular chaperones for folding to the active state. We found that AR(L701H) was highly dependent on Hsp90 for its hormone-independent activation, suggesting that this chaperone functions in AR(L701H) folding. However, the mutant did not respond specifically to increased levels of FKBP52, suggesting that this chaperone functions at the hormone-dependent activation stage in the folding process. Further studies of AR(L701H) in PC3 cells suggested that this mutant is prohibited from hormone-independent transactivation in mammalian cells. However, basal expression of a reporter gene by AR(L701H) was not impaired by the presence of 17-allylamino-17-demethoxygeldanamycin as was wild type AR, suggesting differential interactions of these receptors with molecular chaperones in animal cells.

Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor. Publishing Authors By Initials

K Robzyk,H Oen,G Buchanan,LM Butler,WD Tilley,AK Mandal,N Rosen,AJ Caplan,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

PUBMED ID PMID:

MEDLINE DATE:

Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular and cellular endocrinology

VOLUME: 268

Page Numbers: 67-74

Journal Abbreviation: Mol. Cell. Endocrinol.

ISSN: 0303-7207

DAY: 6

MONTH: 02

YEAR: 2007

Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7500844

Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: genetics

Chemical & Substance for Abstract: Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor. Information

Substance Name: Histidine

Registry Number: 71-00-1

Grant and Affiliation Information for Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor.

AFFILIATION: Department of Medicine and Program in Molecular Pharmacology and Chemistry, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Country: Ireland

AGENCY: United States NIGMS

GRANT: R01 GM070596-03

ACRONYM: GM

MEDLINETA: Mol Cell Endocrinol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor Related Publications

• Adult mesenchymal stem cells for tissue engineering versus regenerative medicine.
• Akt shows variable sensitivity to an Hsp90 inhibitor depending on cell context.
• Aphasic deficits in syntactic processing.
• Arrestins and spinophilin competitively regulate Na+,K+-ATPase trafficking through association with a large cytoplasmic loop of the Na+,K+-ATPase.
• Art, constructional apraxia, and the brain.
• Attack of the anti-cloners.
• Bioactive surface for neural electrodes: decreasing astrocyte proliferation via transforming growth factor-beta1.
• Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation.
• Dizziness: how do patients describe dizziness and how do emergency physicians use these descriptions for diagnosis?
• EHD1 and Eps15 interact with phosphatidylinositols via their Eps15 homology domains.
• EHD1 regulates beta1 integrin endosomal transport: effects on focal adhesions, cell spreading and migration.
• EHD1 regulates cholesterol homeostasis and lipid droplet storage.
• Frontal and temporal volumes in children with epilepsy.
• Hippocampal volume in childhood complex partial seizures.
• In vitro assessment of cone beam local computed tomography for proximal caries detection.
• Inhibition of nuclear factor-kappaB ameliorates bowel injury and prolongs survival in a neonatal rat model of necrotizing enterocolitis.
• Isolation of human marrow-derived mesenchymal stem cells.
• Isolation of rat marrow-derived mesenchymal stem cells.
• Latest developments in collateral circulation, pediatric stroke, and stroke recovery.
• MAL decreases the internalization of the aquaporin-2 water channel.
• Middle cerebral artery occlusive disease: The New England medical center stroke registry.
• Paradoxical clustering of brain metastases in an underperfused cerebral hemisphere.
• Photoacoustic spectroscopy of purple membrane fragments evidence for disorder during photocycle.
• Social difficulties in children with epilepsy: review and treatment recommendations.
• Stimulatory effects of basic fibroblast growth factor and bone morphogenetic protein-2 on osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells.
• Teach your children well. . . .
• Tentacle probes: eliminating false positives without sacrificing sensitivity.
• Transport protein sorting in polarized epithelial cells.
• Uncoupling of hormone-dependence from chaperone-dependence in the L701H mutation of the androgen receptor.
• Unmet mental health needs in pediatric epilepsy: Insights from providers.