Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds.

Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds. Research Abstract Details 

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Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds. Abstract Text:

Manu Forero,Olga Yakovenko,Evgeni V Sokurenko,Wendy E Thomas,Viola Vogel,

We determined whether the molecular structures through which force is applied to receptor-ligand pairs are tuned to optimize cell adhesion under flow. The adhesive tethers of our model system, Escherichia coli, are type I fimbriae, which are anchored to the outer membrane of most E. coli strains. They consist of a fimbrial rod (0.3-1.5 microm in length) built from a helically coiled structural subunit, FimA, and an adhesive subunit, FimH, incorporated at the fimbrial tip. Previously reported data suggest that FimH binds to mannosylated ligands on the surfaces of host cells via catch bonds that are enhanced by the shear-originated tensile force. To understand whether the mechanical properties of the fimbrial rod regulate the stability of the FimH-mannose bond, we pulled the fimbriae via a mannosylated tip of an atomic force microscope. Individual fimbriae rapidly elongate for up to 10 microm at forces above 60 pN and rapidly contract again at forces below 25 pN. At intermediate forces, fimbriae change length more slowly, and discrete 5.0 +/- 0.3-nm changes in length can be observed, consistent with uncoiling and coiling of the helical quaternary structure of one FimA subunit at a time. The force range at which fimbriae are relatively stable in length is the same as the optimal force range at which FimH-mannose bonds are longest lived. Higher or lower forces, which cause shorter bond lifetimes, cause rapid length changes in the fimbria that help maintain force at the optimal range for sustaining the FimH-mannose interaction. The modulation of force and the rate at which it is transmitted from the bacterial cell to the adhesive catch bond present a novel physiological role for the fimbrial rod in bacterial host cell adhesion. This suggests that the mechanical properties of the fimbrial shaft have codeveloped to optimize the stability of the terminal adhesive under flow.

Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds. Publishing Authors By Initials

M Forero,O Yakovenko,EV Sokurenko,WE Thomas,V Vogel,

For similar biomechanics: stress, mechanical research abstracts see: biomechanics: stress, mechanical research

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Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: PLoS biology

VOLUME: 4

Page Numbers: e298

Journal Abbreviation: PLoS Biol.

ISSN: 1545-7885

DAY: 3

MONTH: Sep

YEAR: 2006

Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101183755

Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds. Keywords Mesh Terms:

KEYWORDS: Stress, Mechanical

MESH TERMS: methods

Chemical & Substance for Abstract: Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds. Information

Substance Name: Mannose

Registry Number: 31103-86-3

Grant and Affiliation Information for Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds.

AFFILIATION: Department of Materials, Laboratory for Biologically Oriented Materials, ETH Zurich, Zurich, Switzerland.

Country: United States

AGENCY: United States PHS

GRANT: A150940

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MEDLINETA: PLoS Biol

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