UNC-1 regulates gap junctions important to locomotion in C. elegans.

UNC-1 regulates gap junctions important to locomotion in C. elegans. Research Abstract Details 

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UNC-1 regulates gap junctions important to locomotion in C. elegans. Abstract Text:

Kenneth R Norman,Andres Villu Maricq,Kenneth R Norman,Andres Villu Maricq,

In C. elegans, loss-of-function (lf) mutations of the stomatin-like protein (SLP) UNC-1 and the innexin UNC-9 inhibit locomotion [1, 2] and modulate sensitivity to volatile anesthetics [3, 4]. It was unknown why unc-1(lf) and unc-9(lf) mutants have similar phenotypes. We tested the hypothesis that UNC-1 is a regulator of gap junctions formed by UNC-9. Analyses of junctional currents between body-wall muscle cells showed that electrical coupling was inhibited to a similar degree in unc-1(lf), unc-9(lf), and unc-1(lf);unc-9(lf) double mutants, suggesting that UNC-1 and UNC-9 function together. Expression of Punc-1::DsRED2 and Punc-9::GFP transcriptional fusions suggests that unc-1 and unc-9 are coexpressed in neurons and body-wall muscle cells. Immunohistochemistry showed that UNC-1 and UNC-9 colocalized at intercellular junctions and that unc-1(lf) did not alter UNC-9 expression or subcellular localization. Bimolecular fluorescence complementation (BiFC) assays suggest that UNC-1 and UNC-9 are physically very close at intercellular junctions. Targeted rescue experiments suggest that UNC-9 and UNC-1 function predominantly in neurons to control locomotion. Thus, in addition to the recently reported function of regulating mechanosensitive ion channels [5, 6], SLPs might have a novel function of regulating gap junctions.

UNC-1 regulates gap junctions important to locomotion in C. elegans. Publishing Authors By Initials

KR Norman,AV Maricq,KR Norman,AV Maricq,

For similar nervous system: neurons research abstracts see: nervous system: neurons research

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UNC-1 regulates gap junctions important to locomotion in C. elegans. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Current biology : CB

VOLUME: 17

Page Numbers: 1334-9

Journal Abbreviation: Curr. Biol.

ISSN: 0960-9822

DAY: 19

MONTH: 07

YEAR: 2007

UNC-1 regulates gap junctions important to locomotion in C. elegans. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9107782

UNC-1 regulates gap junctions important to locomotion in C. elegans. Keywords Mesh Terms:

KEYWORDS: Neurons

MESH TERMS: physiology

Chemical & Substance for Abstract: UNC-1 regulates gap junctions important to locomotion in C. elegans. Information

Substance Name: Unc-9 protein, C elegans

Registry Number: 0

Grant and Affiliation Information for UNC-1 regulates gap junctions important to locomotion in C. elegans.

AFFILIATION: Department of Neuroscience, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030.

Country: England

AGENCY: United States NIDA

GRANT: DA 015464

ACRONYM: DA

MEDLINETA: Curr Biol

REFSOURCE: Curr Biol. 2007 Sep 18;17(18):R812-4

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