Ultrasound at 27 kHz increases tissue expression and activity of nitric oxide synthases in acute limb ischemia in rabbits.

Ultrasound at 27 kHz increases tissue expression and activity of nitric oxide synthases in acute limb ischemia in rabbits. Research Abstract Details 

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Ultrasound at 27 kHz increases tissue expression and activity of nitric oxide synthases in acute limb ischemia in rabbits. Abstract Text:

Shaul Atar,Robert J Siegel,Rami Akel,Yumei Ye,Yu Lin,Shreyas A Modi,Asif Sewani,Enrique Tuero,Yochai Birnbaum,

Transcutaneous low-frequency ultrasound (US) preserves myocardial and skeletal muscle viability by increasing tissue perfusion through an undefined nitric oxide (NO)-dependent mechanism. We have examined whether US increases tissue expression and activity of the three nitric oxide synthase (NOS) isoforms: endothelial (eNOS), neuronal (nNOS) and inducible (iNOS). The two femoral arteries of four New Zealand rabbits were ligated for a total of 120 min. After 60 min of ligation, transcutaneous low-frequency US (27 kHz, 0.13 W/cm2) was applied for 60 min to one thigh, while the contra-lateral artery served as a control (total ischemia time=120 min). Calcium-dependent (cNOS) and -independent (ciNOS) NOS activity, and concentration of total eNOS, ser-1177 phosphorylated eNOS (P-eNOS), nNOS and iNOS were then determined in the gracilis muscle. Compared with the control, US application significantly increased cNOS activity [3.34+/-0.28 versus 3.87+/-0.10x1000 counts per minute (cpm), respectively, p=0.031] and ciNOS activity (1.99+/-0.09 versus 3.26+/-0.68 cpm, respectively, p<0.001). Western immunoblotting revealed a significant increase in protein content of both iNOS (184.5+/-1.08%; p<0.0001) and P-eNOS (381.5+/-2.47%; p<0.001), with only a small increase in total eNOS and nNOS expression. In conclusion, application of transcutaneous low-frequency US to ischemic muscular tissue significantly increases both cNOS and ciNOS activity by increasing eNOS phosphorylation and iNOS expression, respectively.

Ultrasound at 27 kHz increases tissue expression and activity of nitric oxide synthases in acute limb ischemia in rabbits. Publishing Authors By Initials

S Atar,RJ Siegel,R Akel,Y Ye,Y Lin,SA Modi,A Sewani,E Tuero,Y Birnbaum,

For similar therapeutics: hyperthermia, induced: diathermy: ultrasonic therapy research abstracts see: therapeutics: hyperthermia, induced: diathermy: ultrasonic therapy research

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Ultrasound at 27 kHz increases tissue expression and activity of nitric oxide synthases in acute limb ischemia in rabbits. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Ultrasound in medicine & biology

VOLUME: 33

Page Numbers: 1483-8

Journal Abbreviation:

ISSN: 0301-5629

DAY: 16

MONTH: 05

YEAR: 2007

Ultrasound at 27 kHz increases tissue expression and activity of nitric oxide synthases in acute limb ischemia in rabbits. Information

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LANGUAGE: eng

NlmUniqueID: 410553

Ultrasound at 27 kHz increases tissue expression and activity of nitric oxide synthases in acute limb ischemia in rabbits. Keywords Mesh Terms:

KEYWORDS: Ultrasonic Therapy

MESH TERMS: methods

Chemical & Substance for Abstract: Ultrasound at 27 kHz increases tissue expression and activity of nitric oxide synthases in acute limb ischemia in rabbits. Information

Substance Name: Nitric Oxide Synthase Type III

Registry Number: EC 1.14.13.39

Grant and Affiliation Information for Ultrasound at 27 kHz increases tissue expression and activity of nitric oxide synthases in acute limb ischemia in rabbits.

AFFILIATION: Division of Cardiology, University of Texas Medical Branch, Galveston, TX 77555-0553, USA.

Country: England

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MEDLINETA: Ultrasound Med Biol

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