Ultrasensitive detection of cortisol with enzyme fragment complementation technology using functionalized nanowire.

Ultrasensitive detection of cortisol with enzyme fragment complementation technology using functionalized nanowire. Research Abstract Details 

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Ultrasensitive detection of cortisol with enzyme fragment complementation technology using functionalized nanowire. Abstract Text:

Arun Kumar,Shyam Aravamudhan,Milorad Gordic,Shekhar Bhansali,Shyam S Mohapatra,

Cortisol is a member of the glucocorticoid hormone family and a key metabolic regulator. Increased intracellular cortisol levels have been implicated in type 2 diabetes, obesity, and metabolic syndrome. Cortisol is an important bio-marker of stress and its detection is also important in sports medicine. However, rapid methods for sensitive detection of cortisol are limited. Functionalized gold nanowires were used to enhance the sensitivity and selectivity of cortisol detection. Gold nanowires are used to improve the electron transfer between the electrodes. Moreover, the large surface to volume ratio, small diffusion time and high electrical conductivity and their aligned nature will enhance the sensitivity and detection limit of the biosensor several fold. The biosensor was fabricated using, aligned gold (Au) nanowires to behave as the working electrode, platinum deposited on a silicon chip to function as the counter electrode, and silver/silver chloride as reference electrode. The gold nanowires were coupled with cortisol antibodies using covalent linkage chemistry and a fixed amount of 3alpha-hydroxysteroid dehydrogenase was introduced into the reaction cell during each measurement to convert (reduce) ketosteroid into hydroxyl steroid. Furthermore, the micro-fluidic, micro-fluid part of the sensor was fabricated using micro-electro-mechanical system (MEMS) technology to have better control on liquid flow over Au nanowires to minimize the signal to noise ratio. The biosensor was characterized using SEM, AFM and FTIR technique. The response curve of the biosensor was found to be linear in the range of 10-80 microM of cortisol. Moreover, the presence of hydrocortisone is sensitively detected in the range of 5-30 microM. It is concluded that the functionalized gold nanowires with micro-fluidic device using enzyme fragment complementation technology can provide an easy and sensitive assay for cortisol detection in serum and other biological fluids.

Ultrasensitive detection of cortisol with enzyme fragment complementation technology using functionalized nanowire. Publishing Authors By Initials

A Kumar,S Aravamudhan,M Gordic,S Bhansali,SS Mohapatra,

For similar technology, industry, and agriculture: manufactured materials: nanostructures: nanowires research abstracts see: technology, industry, and agriculture: manufactured materials: nanostructures: nanowires research

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MEDLINE DATE:

Ultrasensitive detection of cortisol with enzyme fragment complementation technology using functionalized nanowire. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biosensors & bioelectronics

VOLUME: 22

Page Numbers: 2138-44

Journal Abbreviation:

ISSN: 0956-5663

DAY: 9

MONTH: 11

YEAR: 2006

Ultrasensitive detection of cortisol with enzyme fragment complementation technology using functionalized nanowire. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9001289

Ultrasensitive detection of cortisol with enzyme fragment complementation technology using functionalized nanowire. Keywords Mesh Terms:

KEYWORDS: Nanowires

MESH TERMS: instrumentation

Chemical & Substance for Abstract: Ultrasensitive detection of cortisol with enzyme fragment complementation technology using functionalized nanowire. Information

Substance Name: 3-alpha-Hydroxysteroid Dehydrogenase (B-

Registry Number: EC 1.1.1.50

Grant and Affiliation Information for Ultrasensitive detection of cortisol with enzyme fragment complementation technology using functionalized nanowire.

AFFILIATION: Department of Internal Medicine, Division of Allergy and Clinical Immunology, College of Medicine, University of South Florida, Tampa, FL 33612, USA.

Country: England

AGENCY: United States NHLBI

GRANT: HL071110-02

ACRONYM: HL

MEDLINETA: Biosens Bioelectron

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