UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing.

UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. Research Abstract Details 

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UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. Abstract Text:

T Katsube,Y Kazuta,H Mori,K Nakano,K Tanizawa,T Fukui,

We have isolated a cDNA encoding UDP-glucose pyrophosphorylase from a cDNA library of immature potato tuber using oligonucleotide probes synthesized on the basis of partial amino acid sequences of the enzyme. The cDNA clone contained a 1,758-base-pair insert including the complete message for UDP-glucose pyrophosphorylase with 1,431 base pairs. The amino acid sequence of the enzyme inferred from the nucleotide sequence consists of 477 amino acid residues. All the partial amino acid sequences determined protein-chemically [Nakano et al. (1989) J. Biochem. 106, 528-532] confirmed the primary structure of the enzyme. An N-terminal-blocked peptide was isolated from the proteolytic digest of the enzyme protein, and the blocking group was deduced to be an acetyl group by fast atom bombardment-mass spectrometry. On the basis of the predicted amino acid sequence (477 residues minus the N-terminal Met plus an acetyl group), the molecular weight of the enzyme monomer is calculated to be 51,783, which agrees well with the value determined by polyacrylamide gel electrophoresis. In the cDNA structure, the open-reading frame is preceded by a 125-base-pair noncoding region, which contains a sequence being homologous with the consensus sequence for plant genes, and is followed by a 174-base-pair noncoding sequence including a polyadenylation signal. Amino acid sequence comparisons revealed that the potato UDP-glucose pyrophosphorylase is homologous to the enzyme from slime mold, Dictyostelium discoideum, but not to ADP-glucose pyrophosphorylases from rice seed and Escherichia coli.

UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. Publishing Authors By Initials

T Katsube,Y Kazuta,H Mori,K Nakano,K Tanizawa,T Fukui,

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UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 108

Page Numbers: 321-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1990

UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. Keywords Mesh Terms:

KEYWORDS: UTP-Glucose-1-Phosphate Uridylyltransfer

MESH TERMS: genetics

Chemical & Substance for Abstract: UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. Information

Substance Name: Endopeptidases

Registry Number: EC 3.4.-

Grant and Affiliation Information for UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing.

AFFILIATION: Institute of Scientific and Industrial Research, Osaka University.

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: D00667

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