UBP43 (USP18) specifically removes ISG15 from conjugated proteins.

UBP43 (USP18) specifically removes ISG15 from conjugated proteins. Research Abstract Details 

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UBP43 (USP18) specifically removes ISG15 from conjugated proteins. Abstract Text:

UBP43 shows significant homology to well characterized ubiquitin-specific proteases and previously was shown to hydrolyze ubiquitin-beta-galactosidase fusions in Escherichia coli. In our assays, the activity of UBP43 toward Ub fusions was undetectable in vitro directing us to investigate the possibility of Ub-like proteins such as SUMO, Nedd8, and ISG15 as probable substrates. We consequently demonstrate that UBP43 can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. In addition to commonly used methods we introduce a new experimental design featuring ISG15-UBP43 fusion self-processing. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first bona fide ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. We postulate that UBP43 is necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

UBP43 (USP18) specifically removes ISG15 from conjugated proteins. Publishing Authors By Initials

For similar proteins: ubiquitins research abstracts see: proteins: ubiquitins research

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UBP43 (USP18) specifically removes ISG15 from conjugated proteins. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 277

Page Numbers: 9976-81

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 11

MONTH: 01

YEAR: 2002

UBP43 (USP18) specifically removes ISG15 from conjugated proteins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

UBP43 (USP18) specifically removes ISG15 from conjugated proteins. Keywords Mesh Terms:

KEYWORDS: Ubiquitins

MESH TERMS: analogs & derivatives

Chemical & Substance for Abstract: UBP43 (USP18) specifically removes ISG15 from conjugated proteins. Information

Substance Name: Usp18 protein, mouse

Registry Number: EC 3.4.99.-

Grant and Affiliation Information for UBP43 (USP18) specifically removes ISG15 from conjugated proteins.

AFFILIATION: Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA79849

ACRONYM: CA

MEDLINETA: J Biol Chem

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