Ubiquity of parasporin-1 producers in Bacillus thuringiensis natural populations of Japan.

Ubiquity of parasporin-1 producers in Bacillus thuringiensis natural populations of Japan. Research Abstract Details 

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Ubiquity of parasporin-1 producers in Bacillus thuringiensis natural populations of Japan. Abstract Text:

Akiko Uemori,Minoru Maeda,Koichi Yasutake,Akira Ohgushi,Kumiko Kagoshima,Eiichi Mizuki,Michio Ohba,Akiko Uemori,Minoru Maeda,Koichi Yasutake,Akira Ohgushi,Kumiko Kagoshima,Eiichi Mizuki,Michio Ohba,Akiko Uemori,Minoru Maeda,Koichi Yasutake,Akira Ohgushi,Kumiko Kagoshima,Eiichi Mizuki,Michio Ohba,

Parasporin, a Bacillus thuringiensis parasporal protein, is unique in having a strong cytocidal activity preferential for human cancer cells. In this study, we characterized parasporin activities associated with three novel geographical isolates of B. thuringiensis. Parasporal inclusion proteins of the three isolates were highly toxic to human uterus cervix cancer cells (HeLa), but not to non-cancer uterine smooth muscle cells (UtSMC). Inclusions of the isolates lacked insect toxicity and hemolytic activity against sheep erythrocytes. Ouchterlony immunodiffusion tests revealed that the proteins of the three isolates are immunologically closely related to parasporin-1 (Cry31A), but dissimilar to the three other existing parasporin groups. Our results provide evidence that the parasporin-1-producing organism is a common member in B. thuringiensis populations occurring in natural environments of Japan.

Ubiquity of parasporin-1 producers in Bacillus thuringiensis natural populations of Japan. Publishing Authors By Initials

A Uemori,M Maeda,K Yasutake,A Ohgushi,K Kagoshima,E Mizuki,M Ohba,A Uemori,M Maeda,K Yasutake,A Ohgushi,K Kagoshima,E Mizuki,M Ohba,A Uemori,M Maeda,K Yasutake,A Ohgushi,K Kagoshima,E Mizuki,M Ohba,

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Ubiquity of parasporin-1 producers in Bacillus thuringiensis natural populations of Japan. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Die Naturwissenschaften

VOLUME: 94

Page Numbers: 34-8

Journal Abbreviation: Naturwissenschaften

ISSN: 0028-1042

DAY: 7

MONTH: 09

YEAR: 2006

Ubiquity of parasporin-1 producers in Bacillus thuringiensis natural populations of Japan. Information

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LANGUAGE: eng

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AFFILIATION: Graduate School of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan, ohba@brs.kyushu-u.ac.jp.

Country: Germany

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MEDLINETA: Naturwissenschaften

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