Type IV collagen alpha-chain composition in synovial lining from trauma patients and patients with rheumatoid arthritis.

Type IV collagen alpha-chain composition in synovial lining from trauma patients and patients with rheumatoid arthritis. Research Abstract Details 

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Type IV collagen alpha-chain composition in synovial lining from trauma patients and patients with rheumatoid arthritis. Abstract Text:

P Poduval,T Sillat,A Beklen,V P Kouri,I Virtanen,Y T Konttinen,P Poduval,T Sillat,A Beklen,V P Kouri,I Virtanen,Y T Konttinen,P Poduval,T Sillat,A Beklen,V P Kouri,I Virtanen,Y T Konttinen,

OBJECTIVE: Normal synovial lining is composed of macrophage-like type A and fibroblast-like type B lining cells. This sheet-like structure lacks a basement membrane, but its intercellular substance contains some basement membrane components, including type IV collagen. We undertook this study to determine the alpha-chain composition of type IV collagen in normal and arthritic synovial lining, using monoclonal alpha-chain antibodies. METHODS: Samples were analyzed using avidin-biotin-peroxidase complex staining for the presence of collagen alpha1/2(IV), alpha3(IV), alpha4(IV), alpha5(IV), alpha6(IV), matrix metalloproteinase 2 (MMP-2), and MMP-9, and the enzyme activity was detected using gelatin zymography. Double immunofluorescence was performed for type IV collagen/MMP-9 and type IV collagen/CD68. Synovial fibroblasts were studied using quantitative reverse transcriptase-polymerase chain reaction. RESULTS: In mildly inflamed synovium from 5 trauma patients, alpha1/2(IV) chains were strongly stained, but alpha5(IV) and alpha6(IV) chains were weakly stained. Coding messenger RNA was shown in cultured synovial fibroblasts. Basement membranes of blood vessels contained all alpha(IV) chains and served as useful positive sample controls. In the synovial lining from 5 patients with rheumatoid arthritis (RA), all alpha-chains were absent/very weakly stained. This was coupled with numerous type A lining cells containing MMP-9 (type IV collagenase), also found in synovial fluid. CONCLUSION: Synovial lining has a unique and very limited alpha-chain composition, different from that of the vascular basement membrane, which contains all alpha-chains. This special composition and lack of nidogen are probably of relevance for the bidirectional translining diffusion. Such tentative alpha-chain-dependent adhesive and transport-regulating properties seem to be deranged in RA, probably in part due to type IV collagenases produced in the lining and/or released by transmigrating or synovial fluid neutrophils.

Type IV collagen alpha-chain composition in synovial lining from trauma patients and patients with rheumatoid arthritis. Publishing Authors By Initials

P Poduval,T Sillat,A Beklen,VP Kouri,I Virtanen,YT Konttinen,P Poduval,T Sillat,A Beklen,VP Kouri,I Virtanen,YT Konttinen,P Poduval,T Sillat,A Beklen,VP Kouri,I Virtanen,YT Konttinen,

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Type IV collagen alpha-chain composition in synovial lining from trauma patients and patients with rheumatoid arthritis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Arthritis and rheumatism

VOLUME: 56

Page Numbers: 3959-67

Journal Abbreviation: Arthritis Rheum.

ISSN: 0004-3591

DAY: 17

MONTH: Dec

YEAR: 2007

Type IV collagen alpha-chain composition in synovial lining from trauma patients and patients with rheumatoid arthritis. Information

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LANGUAGE: eng

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AFFILIATION: University of Helsinki, Helsinki, Finland.

Country: United States

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MEDLINETA: Arthritis Rheum

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