Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells.

Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells. Research Abstract Details 

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Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells. Abstract Text:

In Young Kim,Seo-Jin Jeong,Eun-Sook Kim,Seung Hee Kim,Aree Moon,In Young Kim,Seo-Jin Jeong,Eun-Sook Kim,Seung Hee Kim,Aree Moon,In Young Kim,Seo-Jin Jeong,Eun-Sook Kim,Seung Hee Kim,Aree Moon,

Tumor cell invasion and metastasis are often associated with matrix metalloproteinases (MMPs), among which MMP-2 and MMP-9 are of central importance. We previously showed that H-Ras, but not N-Ras, induced invasion of MCF10A human breast epithelial cells in which the enhanced expression of MMP-2 was involved. MMP-2 is produced as a latent pro-MMP-2 (72 kDa) to be activated resulting the 62 kDa active MMP-2. The present study investigated if H-Ras and/or N-Ras induces pro-MMP-2 activation of MCF10A cells when cultured in two-dimensional gel of type I collagen. Type I collagen induced activation of pro-MMP-2 only in H-Ras MCF10A cells but not in N-Ras MCF10A cells. Induction of active MMP-2 by type I collagen was suppressed by blocking integrin alpha2, indicating the involvement of integrin signaling in pro-MMP-2 activation. Membrane-type (MT)1-MMP and tissue inhibitor of metalloproteinase (TIMP)-2 were up-regulated by H-Ras but not by N-Ras in the type I collagen-coated gel, suggesting that H-Ras-specific up-regulation of MT1-MMP and TIMP-2 may lead to the activation of pro-MMP-2. Since acquisition of pro-MMP-2 activation can be associated with increased malignant progression, these results may help understanding the mechanisms for the cell surface matrix-degrading potential which will be crucial to the prognosis and therapy of breast cancer metastasis.

Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells. Publishing Authors By Initials

IY Kim,SJ Jeong,ES Kim,SH Kim,A Moon,IY Kim,SJ Jeong,ES Kim,SH Kim,A Moon,IY Kim,SJ Jeong,ES Kim,SH Kim,A Moon,

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Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry and molecular biology

VOLUME: 40

Page Numbers: 825-31

Journal Abbreviation: J. Biochem. Mol. Biol.

ISSN: 1225-8687

DAY: 30

MONTH: Sep

YEAR: 2007

Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells. Information

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LANGUAGE: eng

NlmUniqueID: 9702084

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Grant and Affiliation Information for Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells.

AFFILIATION: College of Pharmacy, Duksung Women's University, Seoul 132-714, Korea.

Country: Korea (South)

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MEDLINETA: J Biochem Mol Biol

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