Two X family DNA polymerases, lambda and mu, in meiotic tissues of the basidiomycete, Coprinus cinereus.

Two X family DNA polymerases, lambda and mu, in meiotic tissues of the basidiomycete, Coprinus cinereus. Research Abstract Details 

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Two X family DNA polymerases, lambda and mu, in meiotic tissues of the basidiomycete, Coprinus cinereus. Abstract Text:

Aiko Sakamoto,Kazuki Iwabata,Akiyo Koshiyama,Hiroko Sugawara,Takuro Yanai,Yoshihiro Kanai,Ryo Takeuchi,Yoko Daikuhara,Yoichi Takakusagi,Kengo Sakaguchi,

The X family DNA polymerases lambda (CcPollambda) and mu (CcPolmu) were shown to be expressed during meiotic prophase in the basidiomycete, Coprinus cinereus. These two polymerases are the only members of the X family in the C. cinereus genome. The open reading frame of CcPollambda encoded a predicted product of 800 amino acid residues and that of CcPolmu of 621 amino acid residues. Both CcPollambda and CcPolmu required Mn(2+) ions for activity, and both were strongly inhibited by dideoxythymidine triphosphate. Unlike their mammalian counterparts, CcPollambda and CcPolmu had no terminal deoxynucleotidyl transferase activity. Immunostaining analysis revealed that CcPollambda was present at meiotic prophase nuclei in zygotene and pachytene cells, which is the period when homologous chromosomes pair and recombine. CcPolmu was present in a slightly wider range of cell stages, zygotene to diplotene. In analyses using D-loop recombination intermediate substrates, we found that both CcPollambda and CcPolmu could promote primer extension of an invading strand in a D-loop structure. Moreover, both polymerases could fully extend the primer in the D-loop substrate, suggesting that D-loop extension is an activity intrinsic to CcPollambda and CcPolmu. Based on these data, we discuss the possible roles of these polymerases in meiosis.

Two X family DNA polymerases, lambda and mu, in meiotic tissues of the basidiomycete, Coprinus cinereus. Publishing Authors By Initials

A Sakamoto,K Iwabata,A Koshiyama,H Sugawara,T Yanai,Y Kanai,R Takeuchi,Y Daikuhara,Y Takakusagi,K Sakaguchi,

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Two X family DNA polymerases, lambda and mu, in meiotic tissues of the basidiomycete, Coprinus cinereus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Chromosoma

VOLUME: 116

Page Numbers: 545-56

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ISSN: 0009-5915

DAY: 2

MONTH: 09

YEAR: 2007

Two X family DNA polymerases, lambda and mu, in meiotic tissues of the basidiomycete, Coprinus cinereus. Information

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LANGUAGE: eng

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AFFILIATION: Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken, 278-8510, Japan, kengo@rs.noda.tus.ac.jp.

Country: Germany

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MEDLINETA: Chromosoma

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