Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation.

Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation. Abstract Text:

Saumendra N Sarkar,Christopher P Elco,Kristi L Peters,Saurabh Chattopadhyay,Ganes C Sen,Saumendra N Sarkar,Christopher P Elco,Kristi L Peters,Saurabh Chattopadhyay,Ganes C Sen,

Innate immune response to viral infection is often triggered by Toll-like receptor 3 (TLR3)-mediated signaling by double-stranded (ds) RNA, which culminates in the activation of the transcription factor NF-kappaB and induction of NF-kappaB-driven genes. We demonstrated that dsRNA-induced phosphorylation of two specific tyrosine residues, 759 and 858, of TLR3 was necessary and sufficient for complete activation of the NF-kappaB pathway. When Tyr-759 of TLR3 was mutated, gene induction was inhibited, although NF-kappaB was partially activated. It was released from IkappaB and translocated to the nucleus but failed to bind to the kappaB site of the target A20 gene promoter. This defect could be attributed to incomplete phosphorylation of the RelA (p65) subunit of NF-kappaB, as revealed by two-dimensional gel analyses of p65, isolated from dsRNA-treated cells expressing either wild type TLR3 or the Tyr-759 --> Phe mutant TLR3. Thus, two phosphotyrosine residues of TLR3 activate two distinct pathways, one leading to NF-kappaB release and the other leading to its phosphorylation.

Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation. Publishing Authors By Initials

SN Sarkar,CP Elco,KL Peters,S Chattopadhyay,GC Sen,SN Sarkar,CP Elco,KL Peters,S Chattopadhyay,GC Sen,

For similar amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research

PUBMED ID PMID:

MEDLINE DATE:

Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 3423-7

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 18

MONTH: 12

YEAR: 2006

Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation. Keywords Mesh Terms:

KEYWORDS: Tyrosine

MESH TERMS: physiology

Chemical & Substance for Abstract: Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation. Information

Substance Name: I-kappa B Kinase

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation.

AFFILIATION: Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA68782

ACRONYM: CA

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation Related Publications

• Aerobically derived lactate stimulates revascularization and tissue repair via redox mechanisms.
• Algorithms for mapping of mRNA targets for microRNA.
• Cardiac hypertrophy: mechanisms and therapeutic opportunities.
• Commentary.
• Commentary.
• Cortical discrimination of complex natural stimuli: can single neurons match behavior?
• Distinct induction patterns and functions of two closely related interferon-inducible human genes, ISG54 and ISG56.
• Electroantennogram responses of the potato tuber moth,Phthorimaea operculella (Lepidoptera;Gelichiidae)to plant volatiles.
• Electron hole flow patterns through the RNA-cleaving 8-17 deoxyribozyme yield unusual information about its structure and folding.
• Kaempferol induces apoptosis in glioblastoma cells through oxidative stress.
• Laser microdissection and capture of pure cardiomyocytes and fibroblasts from infarcted heart regions: perceived hyperoxia induces p21 in peri-infarct myocytes.
• MicroRNA in cutaneous wound healing: a new paradigm.
• Microarray analysis of gene expression: considerations in data mining and statistical treatment.
• Mitochondrial nitric oxide synthase.
• Natural vitamin E alpha-tocotrienol: retention in vital organs in response to long-term oral supplementation and withdrawal.
• Network architecture, receptive fields, and neuromodulation: computational and functional implications of cholinergic modulation in primary auditory cortex.
• One-stage correction of recto-vestibular fistula by trans-fistula anorectoplasty (TFARP).
• Phosphorylation of specific serine residues in the PKR activation domain of PACT is essential for its ability to mediate apoptosis.
• Promises and challenges of faith-based AIDS care and support in Mozambique.
• Quantitation of Principal Bufadienolides in Different Cytotypes of Urginea indica.
• Recent progress in the control of aldosterone secretion.
• Regulation of c-Src activity in glutamate-induced neurodegeneration.
• Repeat organization and epigenetic regulation of the DH-Cmu domain of the immunoglobulin heavy-chain gene locus.
• Stat1 required for interferon-inducible but not constitutive responsiveness to extracellular dsRNA.
• Synergistic effect of anionic lipid enhancer and electroosmosis for transcutaneous delivery of insulin.
• Transcriptome analysis of the ischemia-reperfused remodeling myocardium: temporal changes in inflammation and extracellular matrix.
• Transcriptome-wide analysis of blood vessels laser captured from human skin and chronic wound-edge tissue.
• Transnasal Transsphenoidal Endoscopic Repair of CSF Leakage Using Multilayer Acellular Dermis.
• Two tyrosine residues of Toll-like receptor 3 trigger different steps of NF-kappa B activation.
• Vascular expression of germinal ACE fails to maintain normal blood pressure in ACE-/- mice.