Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages.

Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Research Abstract Details 

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Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Abstract Text:

Rushika M Perera,Roberto Zoncu,Louise Lucast,Pietro De Camilli,Derek Toomre,

Phosphoinositides are thought to play an important role in clathrin-coated pit (CCP) dynamics. Biochemical and structural studies have shown a direct interaction of phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] with endocytic clathrin adaptors, whereas functional studies using cell-free systems or intact cells have demonstrated the importance of PI(4,5)P2 synthesis and dephosphorylation in clathrin coating and uncoating, respectively. Furthermore, genetic manipulations of kinases and phosphatases involved in PI(4,5)P2 metabolism result in major defects in synaptic vesicle recycling and other forms of clathrin-dependent endocytosis. However, live imaging studies of these enzymes at CCPs have not been conducted. We have used multicolor total internal reflection fluorescence microscopy (TIRFM) to visualize the spatial-temporal recruitment of synaptojanin 1 (SJ1), a polyphosphoinositide phosphatase, and its binding partner endophilin to CCPs. Strikingly, we observed differential temporal recruitment of the two major SJ1 splice variants to CCPs. The 145-kDa isoform, the predominant isoform expressed in the brain, was rapidly recruited as a "burst," together with endophilin, at a late stage of CCP formation. In contrast, the nonneuronal ubiquitously expressed 170-kDa isoform of SJ1 was present at all stages of CCP formation. These results raise the possibility that dynamic phosphoinositide metabolism may occur throughout the lifetime of a CCP.

Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Publishing Authors By Initials

RM Perera,R Zoncu,L Lucast,P De Camilli,D Toomre,

For similar proteins: protein isoforms research abstracts see: proteins: protein isoforms research

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Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 19332-7

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 8

MONTH: 12

YEAR: 2006

Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Keywords Mesh Terms:

KEYWORDS: Protein Isoforms

MESH TERMS: metabolism

Chemical & Substance for Abstract: Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages. Information

Substance Name: synaptojanin

Registry Number: EC 3.1.3.-

Grant and Affiliation Information for Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages.

AFFILIATION: Department of Cell Biology, Kavli Institute for Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS36251

ACRONYM: NS

MEDLINETA: Proc Natl Acad Sci U S A

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