Two potent alpha3/5 conotoxins from piscivorous Conus achatinus.

Two potent alpha3/5 conotoxins from piscivorous Conus achatinus. Research Abstract Details 

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Two potent alpha3/5 conotoxins from piscivorous Conus achatinus. Abstract Text:

Li Liu,Geoffrey Chew,Edward Hawrot,Chengwu Chi,Chunguang Wang,

Every cone snail produces a mixture of different conotoxins and secretes them to immobilize their prey and predators. alpha3/5 Conotoxins, isolated from fish-hunting cone snails, target muscle nicotinic acetylcholine receptors. The structure and function of alpha3/5 conotoxin from the piscivorous Conus achatinus have not been studied. We synthesized two pentadecamer peptides, Ac1.1a and Ac1.1b, with appropriate disulfide bonding, based on cDNA sequences of alpha3/5 conotoxins from C. achatinus. Ac1.1a and Ac1.1b differ by only one amino acid residue. They have similar potency on blocking recombinant mouse muscle acetylcholine receptor expressed in Xenopus laevis oocytes, with IC50 values of 36 nM and 26 nM, respectively. For Ac1.1b, deletion of the first three N-terminal amino acids did not change its activity, indicating that the N-terminus is not involved in the interaction with its receptor. Furthermore, our experiments indicate that both toxins strongly prefer the alpha1-delta subunit interface instead of the alpha1-gamma binding site on the mouse muscle nicotinic acetylcholine receptor. These peptides provide additional tools for the study of the structure and function of nicotinic receptor.

Two potent alpha3/5 conotoxins from piscivorous Conus achatinus. Publishing Authors By Initials

L Liu,G Chew,E Hawrot,C Chi,C Wang,

For similar animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus research abstracts see: animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus research

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Two potent alpha3/5 conotoxins from piscivorous Conus achatinus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta biochimica et biophysica Sinica

VOLUME: 39

Page Numbers: 438-44

Journal Abbreviation: Acta Biochim. Biophys. Sin. (S

ISSN: 1672-9145

DAY: 3

MONTH: Jun

YEAR: 2007

Two potent alpha3/5 conotoxins from piscivorous Conus achatinus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101206716

Two potent alpha3/5 conotoxins from piscivorous Conus achatinus. Keywords Mesh Terms:

KEYWORDS: Xenopus

MESH TERMS: metabolism

Chemical & Substance for Abstract: Two potent alpha3/5 conotoxins from piscivorous Conus achatinus. Information

Substance Name: Receptors, Nicotinic

Registry Number: 0

Grant and Affiliation Information for Two potent alpha3/5 conotoxins from piscivorous Conus achatinus.

AFFILIATION: Institute of Protein Research, Tongji University, Shanghai 200092, China.

Country: China

AGENCY: United States NIGMS

GRANT: GM32629

ACRONYM: GM

MEDLINETA: Acta Biochim Biophys Sin (Shan

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