We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).
Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source. Publishing Authors By Initials
Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biomedical optics
VOLUME: 12
Page Numbers: 054019
Journal Abbreviation:
ISSN: 1083-3668
DAY: 12
MONTH: 11
YEAR: 2007
Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source. Information
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LANGUAGE: eng
NlmUniqueID: 9605853
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Grant and Affiliation Information for Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source.
AFFILIATION: Tohoku University, New Industry Creation Hatchery Center (NICHe), 6-6-10 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan.
Country: United States
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MEDLINETA: J Biomed Opt
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