Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties.

Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties. Research Abstract Details 

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Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties. Abstract Text:

H Hatanaka,Y Ogawa,F Egami,

Two glycosulfatases [EC 3.1.6.3], I and II, were purified 31.3- and 33.9-fold respectively, from a crude extract of the liver of Charonia lampas. The purification was carried out by the following chromatographic procedures; phosphocellulose, Sephadex G-150, Concanavalin A-Sepharose and isoelectric focussing. The enzyme preparations obtained were practically free from arylsulfatase [EC 3.1.6.1] contamination. Both glycosulfatases are probably glycoproteins differing in their carbohydrate moieties. The molecular weights of glycosulfatase I and II were estimated to be about 112,000 and 79,000 respectively. They had the same optimum pH of 5.5, and the same Km value of 25.0 mM for glucose 6-sulfate.

Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties. Publishing Authors By Initials

H Hatanaka,Y Ogawa,F Egami,

For similar enzymes and coenzymes: enzymes: hydrolases: esterases: sulfatases research abstracts see: enzymes and coenzymes: enzymes: hydrolases: esterases: sulfatases research

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Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 79

Page Numbers: 27-34

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1976

Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties. Keywords Mesh Terms:

KEYWORDS: Sulfatases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties. Information

Substance Name: Sulfatases

Registry Number: EC 3.1.6.-

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Country: JAPAN

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MEDLINETA: J Biochem

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