Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM).

Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM). Research Abstract Details 

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Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM). Abstract Text:

R Babu,S K Nair,A Kumar,S Venkatesh,J C Sekhar,N N Singh,G Srinivasan,H S Gupta,

The low nutritive value of maize endosperm protein is genetically corrected in quality protein maize (QPM), which contains the opaque 2 gene along with numerous modifiers for kernel hardness. We report here a two generation marker-based backcross breeding program for incorporation of the opaque 2 gene along with phenotypic selection for kernel modification in the background of an early maturing normal maize inbred line, V25. Using the flanking marker distances from opaque 2 gene in the cross V 25 xCML 176, optimum population size for the BC(2) generation was computed in such a way that at least one double recombinant could be obtained. Whole genome background selection in the BC(2) generation identified three plants with 93 to 96% recurrent parent genome content. The three BC(2)F(2) families derived from marker identified BC(2) individuals were subjected to foreground selection and phenotypic selection for kernel modification. The tryptophan concentration in endosperm protein was significantly enhanced in all the three classes of kernel modification viz., less than 25%, 25--50% and more than 50% opaqueness. BC(2)F(3) lines developed from the hard endosperm kernels were evaluated for desirable agronomic and biochemical traits in replicated trials and the best line was chosen to represent the QPM version of V25, with tryptophan concentration of 0.85% in protein. The integrated breeding strategy reported here can be applied to reduce genetic drag as well as the time involved in a conventional line conversion program, and would prove valuable in rapid development of specialty corn germ plasm.

Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM). Publishing Authors By Initials

R Babu,SK Nair,A Kumar,S Venkatesh,JC Sekhar,NN Singh,G Srinivasan,HS Gupta,

For similar plants: plant families and groups: angiosperms: poaceae: zea mays research abstracts see: plants: plant families and groups: angiosperms: poaceae: zea mays research

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Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 111

Page Numbers: 888-97

Journal Abbreviation: Theor. Appl. Genet.

ISSN: 0040-5752

DAY: 18

MONTH: 10

YEAR: 2005

Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM). Information

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LANGUAGE: eng

NlmUniqueID: 145600

Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM). Keywords Mesh Terms:

KEYWORDS: Zea mays

MESH TERMS: standards

Chemical & Substance for Abstract: Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM). Information

Substance Name: Genetic Markers

Registry Number: 0

Grant and Affiliation Information for Two-generation marker-aided backcrossing for rapid conversion of normal maize lines to quality protein maize (QPM).

AFFILIATION: Vivekananda Institute of Hill Agriculture (ICAR), Almora, Uttaranchal 263601, India. rbabu_icar@rediffmail.com

Country: Germany

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MEDLINETA: Theor Appl Genet

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