Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies.

Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies. Research Abstract Details 

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Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies. Abstract Text:

Masaru Hamaoki,Kyoko Hiramatsu,Setsuo Suzuki,Atsuo Nagata,Masao Kawakita,

We obtained monoclonal antibodies against N(1),N(12)-diacetylspermine (DiAcSpm) and N(1),N(8)-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these di-acetylpolyamines. Cross-reactions with N(1)-acetylspermidine in the assay of DiAcSpm and with N8-acetylspermidine in the assay of DiAcSpd were as low as 0.26 and 0.6%, respectively, and were judged to be insignificant in clinical use for measuring urinary diacetylpolyamines. These assays were used to assess diurnal variations in diacetylpolyamine excretion in urine to show that the excretion of diacetylpolyamines after normalization for the concentration of creatinine is stable over a day with only minimal diurnal variation.

Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies. Publishing Authors By Initials

M Hamaoki,K Hiramatsu,S Suzuki,A Nagata,M Kawakita,

For similar organic chemicals: amines: biogenic amines: biogenic polyamines: putrescine: spermidine: spermine research abstracts see: organic chemicals: amines: biogenic amines: biogenic polyamines: putrescine: spermidine: spermine research

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Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 132

Page Numbers: 783-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 2002

Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies. Keywords Mesh Terms:

KEYWORDS: Spermine

MESH TERMS: immunology

Chemical & Substance for Abstract: Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies. Information

Substance Name: N',N''-diacetylspermine

Registry Number: 77928-71-3

Grant and Affiliation Information for Two enzyme-linked immunosorbent assay (ELISA) systems for N1,N8-diacetylspermidine and N1,N12-diacetylspermine using monoclonal antibodies.

AFFILIATION: Immunology Laboratory, Diagnostics Department, Yamasa Corporation, Araoi-cho, Choshi, Chiba 288-0056. hamaoki@yamasa.com

Country: Japan

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MEDLINETA: J Biochem

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