Two different ways that hydrogen ions are involved in the thermodynamics and rapid-equilibrium kinetics of the enzymatic catalysis of S=P and S+H2O=P.

Two different ways that hydrogen ions are involved in the thermodynamics and rapid-equilibrium kinetics of the enzymatic catalysis of S=P and S+H2O=P. Research Abstract Details 

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Two different ways that hydrogen ions are involved in the thermodynamics and rapid-equilibrium kinetics of the enzymatic catalysis of S=P and S+H2O=P. Abstract Text:

Robert A Alberty,

Hydrogen ions are involved in two different ways in the thermodynamics and rapid-equilibrium kinetics of enzyme-catalyzed reactions. The two ways are through pKs and through the production or consumption of hydrogen ions in the mechanism. These ways are examined for the catalyzed reactions S=P and S+H2O=P. Since the apparent equilibrium constant K' can be calculated from the kinetic parameters by use of the Haldane equation, the treatment of the effects of pH must be consistent in thermodynamics and kinetics. This leads to a new kind of Haldane equation that involves 10(pH) or 10(-pH) in addition to the kinetic parameters when hydrogen ions are produced or consumed. These concepts are applicable to more complicated reactions and rate equations. Derivations of equations for calculating these two types of pH effects are discussed in thermodynamics and rapid-equilibrium kinetics. A computer program is used to make four plots of apparent equilibrium constants and changes in the binding of hydrogen ions in the catalyzed reaction.

Two different ways that hydrogen ions are involved in the thermodynamics and rapid-equilibrium kinetics of the enzymatic catalysis of S=P and S+H2O=P. Publishing Authors By Initials

RA Alberty,

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Two different ways that hydrogen ions are involved in the thermodynamics and rapid-equilibrium kinetics of the enzymatic catalysis of S=P and S+H2O=P. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biophysical chemistry

VOLUME: 128

Page Numbers: 204-9

Journal Abbreviation: Biophys. Chem.

ISSN: 0301-4622

DAY: 22

MONTH: 04

YEAR: 2007

Two different ways that hydrogen ions are involved in the thermodynamics and rapid-equilibrium kinetics of the enzymatic catalysis of S=P and S+H2O=P. Information

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LANGUAGE: eng

NlmUniqueID: 403171

Two different ways that hydrogen ions are involved in the thermodynamics and rapid-equilibrium kinetics of the enzymatic catalysis of S=P and S+H2O=P. Keywords Mesh Terms:

KEYWORDS: Water

MESH TERMS: chemistry

Chemical & Substance for Abstract: Two different ways that hydrogen ions are involved in the thermodynamics and rapid-equilibrium kinetics of the enzymatic catalysis of S=P and S+H2O=P. Information

Substance Name: Water

Registry Number: 7732-18-5

Grant and Affiliation Information for Two different ways that hydrogen ions are involved in the thermodynamics and rapid-equilibrium kinetics of the enzymatic catalysis of S=P and S+H2O=P.

AFFILIATION: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. alberty@mit.edu

Country: Netherlands

AGENCY: United States NIGMS

GRANT: 5-R01-GM48348-11

ACRONYM: GM

MEDLINETA: Biophys Chem

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