Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis.

Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis. Abstract Text:

James C Charity,Michelle M Costante-Hamm,Emmy L Balon,Dana H Boyd,Eric J Rubin,Simon L Dove,

The MglA protein is the only known regulator of virulence gene expression in Francisella tularensis, yet it is unclear how it functions. F. tularensis also contains an MglA-like protein called SspA. Here, we show that MglA and SspA cooperate with one another to control virulence gene expression in F. tularensis. Using a directed proteomic approach, we show that both MglA and SspA associate with RNA polymerase (RNAP) in F. tularensis, and that SspA is required for MglA to associate with RNAP. Furthermore, bacterial two-hybrid and biochemical assays indicate that MglA and SspA interact with one another directly. Finally, through genome-wide expression analyses, we demonstrate that MglA and SspA regulate the same set of genes. Our results suggest that a complex involving both MglA and SspA associates with RNAP to positively control virulence gene expression in F. tularensis. The F. tularensis genome is unusual in that it contains two genes encoding different alpha subunits of RNAP, and we show here that these two alpha subunits are incorporated into RNAP. Thus, as well as identifying SspA as a second critical regulator of virulence gene expression in F. tularensis, our findings provide a framework for understanding the mechanistic basis for virulence gene control in a bacterium whose transcription apparatus is unique.

Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis. Publishing Authors By Initials

JC Charity,MM Costante-Hamm,EL Balon,DH Boyd,EJ Rubin,SL Dove,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research

PUBMED ID PMID:

MEDLINE DATE:

Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: PLoS pathogens

VOLUME: 3

Page Numbers: e84

Journal Abbreviation: PLoS Pathog.

ISSN: 1553-7374

DAY: 3

MONTH: Jun

YEAR: 2007

Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101238921

Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis. Keywords Mesh Terms:

KEYWORDS: Virulence

MESH TERMS: genetics

Chemical & Substance for Abstract: Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis. Information

Substance Name: DNA-Directed RNA Polymerases

Registry Number: EC 2.7.7.6

Grant and Affiliation Information for Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis.

AFFILIATION: Division of Infectious Diseases, Children's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.

Country: United States

AGENCY: United States NIAID

GRANT: AI069219

ACRONYM: AI

MEDLINETA: PLoS Pathog

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis Related Publications

• A target-selected Apc-mutant rat kindred enhances the modeling of familial human colon cancer.
• A web-based approach to managing stress and mood disorders in the workforce.
• Atm is a negative regulator of intestinal neoplasia.
• Cellular expression patterns of genes upregulated in murine and human colonic neoplasms.
• Complete fractures of the femur in Paget's disease of bone.
• Emergence of class 1 integron-associated GES-5 and GES-5-like extended-spectrum beta-lactamases in clinical isolates of Pseudomonas aeruginosa in South Africa.
• Endocrine function in male and female homosexuals.
• Gene expression of a green fluorescent protein homolog as a host-specific biomarker of heat stress within a reef-building coral.
• Identification of Mom7, a novel modifier of Apc(Min/+) on mouse chromosome 18.
• Local and global regulators linking anaerobiosis to cupA fimbrial gene expression in Pseudomonas aeruginosa.
• Monitoring indoor CO2.
• Niche partitioning of closely related symbiotic dinoflagellates.
• Nifty transport device doubles durability of donor organs.
• Oestrogens in oophorectomized women.
• Pregnancy after liver transplantation.
• President's page: responding to the drum beat for health care reform.
• Progressive scoliosis as the first presenting sign of syringomyelia. Report of a case.
• Role of molecular charge and hydrophilicity in regulating the kinetics of crystal growth.
• The pleiotropic phenotype of apc mutations in the mouse: allele specificity and effects of the genetic background.
• Toaster oven helps researchers toy with microfluidics.
• Transcription terminators in lambda appendix Deletion of control sites in the pL operon of phage lambda.
• Treatment of acute myocardial infarction with intracoronary infusion of streptokinase.
• Twin RNA polymerase-associated proteins control virulence gene expression in Francisella tularensis.
• Widespread hyperplasia induced by transgenic TGF{alpha} in ApcMin mice is associated with only regional effects on tumorigenesis.