Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci.

Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Research Abstract Details 

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Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Abstract Text:

Mandana Veiseh,Patrik Gabikian,S-Bahram Bahrami,Omid Veiseh,Miqin Zhang,Robert C Hackman,Ali C Ravanpay,Mark R Stroud,Yumiko Kusuma,Stacey J Hansen,Deborah Kwok,Nina M Munoz,Raymond W Sze,William M Grady,Norman M Greenberg,Richard G Ellenbogen,James M Olson,

Toward the goal of developing an optical imaging contrast agent that will enable surgeons to intraoperatively distinguish cancer foci from adjacent normal tissue, we developed a chlorotoxin:Cy5.5 (CTX:Cy5.5) bioconjugate that emits near-IR fluorescent signal. The probe delineates malignant glioma, medulloblastoma, prostate cancer, intestinal cancer, and sarcoma from adjacent non-neoplastic tissue in mouse models. Metastatic cancer foci as small as a few hundred cells were detected in lymph channels. Specific binding to cancer cells is facilitated by matrix metalloproteinase-2 (MMP-2) as evidenced by reduction of CTX:Cy5.5 binding in vitro and in vivo by a pharmacologic blocker of MMP-2 and induction of CTX:Cy5.5 binding in MCF-7 cells following transfection with a plasmid encoding MMP-2. Mouse studies revealed that CTX:Cy5.5 has favorable biodistribution and toxicity profiles. These studies show that CTX:Cy5.5 has the potential to fundamentally improve intraoperative detection and resection of malignancies.

Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Publishing Authors By Initials

M Veiseh,P Gabikian,SB Bahrami,O Veiseh,M Zhang,RC Hackman,AC Ravanpay,MR Stroud,Y Kusuma,SJ Hansen,D Kwok,NM Munoz,RW Sze,WM Grady,NM Greenberg,RG Ellenbogen,JM Olson,

For similar arthropod venoms: scorpion venoms research abstracts see: arthropod venoms: scorpion venoms research

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Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cancer research

VOLUME: 67

Page Numbers: 6882-8

Journal Abbreviation: Cancer Res.

ISSN: 0008-5472

DAY: 15

MONTH: Jul

YEAR: 2007

Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2984705

Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Keywords Mesh Terms:

KEYWORDS: Scorpion Venoms

MESH TERMS: chemistry

Chemical & Substance for Abstract: Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Information

Substance Name: Matrix Metalloproteinase 2

Registry Number: EC 3.4.24.24

Grant and Affiliation Information for Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci.

AFFILIATION: Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, 1100 Fairview Avenue N., Seattle, WA 98109, USA.

Country: United States

AGENCY: United States NCI

GRANT: P30 CA 15704-31

ACRONYM: CA

MEDLINETA: Cancer Res

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