Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis.

Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis. Abstract Text:

Charles J Lockwood,Felice Arcuri,Paolo Toti,Claudio De Felice,Graciela Krikun,Seth Guller,Lynn F Buchwalder,Frederick Schatz,

Chorioamnionitis is associated with intense neutrophil infiltration of the decidua. We therefore determined whether chorioamnionitis enhances decidual interleukin-8 (IL-8) expression and examined cytokine-regulated decidual IL-8 expression. Decidua from chorioamnionitis-complicated pregnancies, but not term controls, displayed marked IL-8 immunohistochemical staining and a dense neutrophil infiltrate. Reverse transcriptase-polymerase chain reaction of microdissected decidual cells identified IL-8 mRNA, confirming decidual synthesis of IL-8. Confluent leukocyte-free term decidual cells were primed with 10(-8) mol/L estradiol (E2) or E2 + 10(-7) mol/L medroxyprogesterone acetate to mimic the steroidal milieu of pregnancy. Compared with cultures maintained in E2 alone, E2 + medroxyprogesterone acetate neither significantly affected IL-8 levels nor altered the response to the cytokines. The addition of 1.0 ng/ml tumor necrosis factor-alpha (TNF-alpha) or interleukin-1 beta (IL-1beta) increased IL-8 secretion levels by 236.6 +/- 51.4- and 1062.6 +/- 254.3-fold, respectively (n = 8, mean +/- SEM, P < 0.05), as measured by enzyme-linked immunosorbent assay. Concentration-response studies revealed that 0.01 ng/ml TNF-alpha and IL-1beta elevated IL-8 output by 10- and 100-fold, respectively. Western blotting confirmed these results, and quantitative reverse transcriptase-polymerase chain reaction demonstrated parallel changes in mRNA levels. In conclusion, IL-8 is strongly expressed in term decidua during chorioamnionitis, and TNF-alpha and IL-1beta enhance IL-8 expression in term decidual cells, suggesting that these cytokines are important regulators of chorioamnionitis-related decidual neutrophil infiltration.

Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis. Publishing Authors By Initials

CJ Lockwood,F Arcuri,P Toti,CD Felice,G Krikun,S Guller,LF Buchwalder,F Schatz,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The American journal of pathology

VOLUME: 169

Page Numbers: 1294-302

Journal Abbreviation:

ISSN: 0002-9440

DAY: 3

MONTH: Oct

YEAR: 2006

Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370502

Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis.

AFFILIATION: Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 333 Cedar Street, Room 335 FMB P.O. Box 208063 New Haven, CT. Frederick.Schatz@yale.edu.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Am J Pathol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis Related Publications

• "Taking care of my own blood": older women's relationships to their households in rural South Africa.
• A reinterpretation of the mechanism of the simplest reaction at an sp3-hybridized carbon atom: H + CD4 --> CD3 + HD.
• A survey of the burden of allergic rhinitis in the USA.
• Activation-induced cytidine deaminase-mediated sequence diversification is transiently targeted to newly integrated DNA substrates.
• Automatic generation of steady state enzyme rate equation and the associated constraint equations.
• CONSIDERATIONS ON THE OCCURENCE AND IMPORTANCE OF PARODOXICAL CONCENTRATION PHENOMENA IN BIOLOGICAL SYSTEMS.
• Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences.
• Coping with change: household structure and composition in rural South Africa, 1992 - 2003.
• Crossed-beams and theoretical studies of the O((3)P) + H(2)O --> HO(2) + H reaction excitation function.
• Cytokines and type 1 diabetes complications: casual or causal association?
• Differential regulation of colony stimulating factor 1 and macrophage migration inhibitory factor expression by inflammatory cytokines in term human decidua: implications for macrophage trafficking at the fetal-maternal interface.
• Endocrine complications of childhood cancer therapy: evaluation and management.
• Fluorescence resonance energy transfer analysis of recombination signal sequence configuration in the RAG1/2 synaptic complex.
• Import of proteins into mitochondria and chloroplasts.
• Inducible gene expression using an autoregulatory, tetracycline-controlled system.
• Inducible gene expression using an autoregulatory, tetracycline-controlled system.
• Metamaterials with gradient negative index of refraction.
• Mitochondrial oxidations and energy coupling.
• Persistence of high health care expenditures among children in Medicaid.
• Pharmacotherapy of asthma: What do the 2007 NAEPP guidelines say?
• Resonance vibrational Raman optical activity: a time-dependent density functional theory approach.
• Role of activation-induced deaminase protein kinase A phosphorylation sites in Ig gene conversion and somatic hypermutation.
• Stimulation of thyroid cell growth by thyrotropin and epidermal growth factor in isolated porcine thyroid follicles.
• Streptomycin-induced chlorophyll-less races of Euglena.
• Synthesis of silver nanodisks using polystyrene mesospheres as templates.
• The beyond 12/23 restriction is imposed at the nicking and pairing steps of DNA cleavage during V(D)J recombination.
• The role of decidualization in regulating endometrial hemostasis during the menstrual cycle, gestation, and in pathological states.
• Tumor necrosis factor-alpha and interleukin-1beta regulate interleukin-8 expression in third trimester decidual cells: implications for the genesis of chorioamnionitis.
• Type 1 diabetes intervention trials 2007: where are we and where are we going?
• [Pathogenesis of diabetes mellitus]