Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation.

Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation. Abstract Text:

Meilleur,Casimir D Akpovi,R-Marc Pelletier, Vitale, Meilleur,Casimir D Akpovi,R-Marc Pelletier, Vitale,

The anterior pituitary folliculostellate (FS) cells are key elements of the paracrine control of the pituitary function. These cells are the source and the target of growth factors and cytokines, and are connected to other pituitary cells via Cx43-mediated gap junctions. Here, we show that acute treatment of the FS TtT/GF cell line with TNF-alpha caused a transient cell uncoupling that was accompanied by the dephosphorylation of Cx43 in Ser368. These TNF-alpha-evoked effects were dependent on protein phosphatase 2A (PP2A) and protein kinase C (PKC) activities. TNF-alpha did not affect total cell Cx43-PP2A catalytic subunit interaction, but it did induce PP2A catalytic subunit recruitment to the Triton X-100 insoluble subcellular fraction, in which Cx43-gap junction plaques are recovered. This recruitment temporally coincided with Cx43 phosphorylated in Ser368-Cx43 dephosphorylation. Cx43 did not interact with the conventional PKC-alpha, but it did interact with the atypical PKC-zeta. Moreover, this interaction was weakened by TNF-alpha. Cx43 dephosphorylation in Ser368 was followed by the tyrosine phosphorylation of the protein. The temporary closure of gap junctions during acute TNF-alpha challenge may constitute a protective mechanism to limit or confine the spread of inflammatory signals among the FS cells.

Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation. Publishing Authors By Initials

MA Meilleur,CD Akpovi,RM Pelletier,ML Vitale,MA Meilleur,CD Akpovi,RM Pelletier,ML Vitale,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Endocrinology

VOLUME: 148

Page Numbers: 5913-24

Journal Abbreviation: Endocrinology

ISSN: 0013-7227

DAY: 13

MONTH: 09

YEAR: 2007

Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375040

Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation.

AFFILIATION: Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, 2900 Edouard-Montpetit, Montréal, Québec, Canada H3T 1J4. maria.leiza.vitale@umontreal.ca.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Endocrinology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation Related Publications

• A counter controllable high pressure cloud chamber.
• Brief intervention protocol for assisting patients with tobacco cessation.
• Cerebellar astrocytomas in children.
• Describing measurement uncertainties in room acoustics with the concept of GUM.
• Effects of fluconazole in the prophylaxis of oropharyngeal candidiasis in patients undergoing radiotherapy for head and neck tumour: results from a double-blind placebo-controlled trial.
• Exogenous hydrogen sulfide induces functional inhibition and cell death of cytotoxic lymphocytes subsets.
• For which patients with left main stem disease is percutaneous intervention rather than coronary artery bypass grafting the better option?
• Genetic diversity of HIV-1 non-B strains in Sicily: evidence of intersubtype recombinants by sequence analysis of gag, pol, and env genes.
• Isoform B of myosin II heavy chain mediates actomyosin contractility during TNF{alpha}-induced apoptosis.
• Limited access to care and home healthcare.
• Mortality study of ethylene oxide workers in chemical manufacturing: a 10 year update.
• Multiwavelength sun-photometer calibration corrected on the basis of the spectral features characterizing particulate extinction and nitrogen dioxide absorption.
• Perturbations of natural killer cell regulatory functions in respiratory allergic diseases.
• Phenotypic analysis of receptor-ligand pairs on B-cells in B-chronic lymphocytic leukemia.
• Phospholipase D1 production of phosphatidic acid at the plasma membrane promotes exocytosis of large dense-core granules at a late stage.
• Prevalence and distribution of odontogenic cysts in Sicily: 1986 - 2005.
• SUBACUTE BACTERIAL ENDOCARDITIS COMPLICATING PATENT DUCTUS ARTERIOSUS IN THE AGED.
• Screening for idiopathic scoliosis in adolescents. An information statement.
• Specifically designed physical exercise programs improve children's motor abilities.
• THE EFFECT OF VARIATIONS IN MOTION FIDELITY DURING TRAINING ON SIMULATED LOW-ALTITUDE FLIGHT. TECHN DOCUM REP AMRL-TDR-63-108.
• Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells.
• Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation.
• [Dissecting aneurysms of the aorta. Literature review and presentation of 3 cases in young adults]
• [EEG registration of paroxysmal autonomic crises.]
• [Multiple spinocellular carcinomas of the skin in O.R.L. sites (description of a case)]
• [Not Available.]
• [Prevalence of hepatitis virus A, B, and C markers according to the geographic origin of medical students]
• [Recent data on Rehn test.]
• [Tinea nigra palmaris: a clinical case in Argentina]
• [Waking reaction in the pathological EEG in man.]