Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes.

Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes. Abstract Text:

Katherine S Ralston,Kent L Hill,

The Trypanosoma brucei flagellum is a multifunctional organelle with critical roles in motility, cellular morphogenesis, and cell division. Although motility is thought to be important throughout the trypanosome lifecycle, most studies of flagellum structure and function have been restricted to the procyclic lifecycle stage, and our knowledge of the bloodstream form flagellum is limited. We have previously shown that trypanin functions as part of a flagellar dynein regulatory system that transmits regulatory signals from the central pair apparatus and radial spokes to axonemal dyneins. Here we investigate the requirement for this dynein regulatory system in bloodstream form trypanosomes. We demonstrate that trypanin is localized to the flagellum of bloodstream form trypanosomes, in a pattern identical to that seen in procyclic cells. Surprisingly, trypanin RNA interference is lethal in the bloodstream form. These knockdown mutants fail to initiate cytokinesis, but undergo multiple rounds of organelle replication, accumulating multiple flagella, nuclei, kinetoplasts, mitochondria, and flagellum attachment zone structures. These findings suggest that normal flagellar beat is essential in bloodstream form trypanosomes and underscore the emerging concept that there is a dichotomy between trypanosome lifecycle stages with respect to factors that contribute to cell division and cell morphogenesis. This is the first time that a defined dynein regulatory complex has been shown to be essential in any organism and implicates the dynein regulatory complex and other enzymatic regulators of flagellar motility as candidate drug targets for the treatment of African sleeping sickness.

Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes. Publishing Authors By Initials

KS Ralston,KL Hill,

For similar parasitic diseases: protozoan infections: sarcomastigophora infections: mastigophora infections: trypanosomiasis: trypanosomiasis, african research abstracts see: parasitic diseases: protozoan infections: sarcomastigophora infections: mastigophora infections: trypanosomiasis: trypanosomiasis, african research

PUBMED ID PMID:

MEDLINE DATE:

Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: PLoS pathogens

VOLUME: 2

Page Numbers: e101

Journal Abbreviation: PLoS Pathog.

ISSN: 1553-7374

DAY: 3

MONTH: Sep

YEAR: 2006

Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101238921

Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes. Keywords Mesh Terms:

KEYWORDS: Trypanosomiasis, African

MESH TERMS: pathology

Chemical & Substance for Abstract: Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes. Information

Substance Name: Dynein ATPase

Registry Number: EC 3.6.4.2

Grant and Affiliation Information for Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes.

AFFILIATION: Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California, United States of America.

Country: United States

AGENCY: United States NIAID

GRANT: R01AI52348

ACRONYM: AI

MEDLINETA: PLoS Pathog

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes Related Publications

• Action on cycling in primary care trusts: Results of a survey of Directors of Public Health.
• Apolipoprotein E epsilon4 offers protection against age-related macular degeneration.
• Characterizing electrospray ionization using atmospheric pressure ion mobility spectrometry.
• Cultural competency training for third-year clerkship students: effects of an interactive workshop on student attitudes.
• Does Dixon's Integrative Environmental Health Model inform an understanding of rural parents' perceptions of local environmental health risks?
• Expanding the role of the dynein regulatory complex to non-axonemal functions: association of GAS11 with the Golgi apparatus.
• Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella.
• Gas-phase chiral separations by ion mobility spectrometry.
• Impact of nutritional support on the clinical outcome of the surgical patient.
• Internalization of near-infrared fluorescent dyes within isolated leukocyte populations.
• MUC4 and MUC5B transcripts are the prevalent mucin messenger ribonucleic acids of the human endocervix.
• Male eastern bluebirds trade future ornamentation for current reproductive investment.
• Management of celiac disease in childhood and adolescence: unique challenges and strategies.
• NaTHNaC-the National Travel Health Network and Centre (England).
• Nerve-target interactions in the gustatory system following unilateral chorda tympani nerve section.
• Novel inhibitors of the trypanosome alternative oxidase inhibit Trypanosoma brucei brucei growth and respiration.
• Recommended reading and viewing.
• Some probabilistic results on the nonrandomness of simple sequence repeats in DNA sequences.
• Spectroscopy and electrochemistry of cytochrome P450 BM3-surfactant film assemblies.
• Structure and dynamics of ASC2, a pyrin domain-only protein that regulates inflammatory signaling.
• Structure of ATP-bound human ATP:cobalamin adenosyltransferase.
• Sudden illness in infancy.
• Suppression of endothelial or lipoprotein lipase in THP-1 macrophages attenuates proinflammatory cytokine secretion.
• The SPf66 Malaria Vaccine: Where Do We Go From Here?
• The angiotensin-converting enzyme (ACE) gene insertion/deletion dimorphism tracks with higher serum ace activities in both younger and older subjects.
• The complete cDNA sequence and structural polymorphism of the polypeptide chain of porcine submaxillary mucin.
• Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes.
• Two-month follow-up on callers to a telephone quit smoking service.
• Underserved urban african american men: hypertension trial outcomes and mortality during 5 years.
• Wireless Surgical Tools for Mechanical Measurements during Scoliosis Surgery.