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Tropomyosin isoforms in developing chicken gizzard smooth muscle.

Tropomyosin isoforms in developing chicken gizzard smooth muscle. Research Abstract Details 

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  • Tropomyosin isoforms in developing chicken gizzard smooth muscle. Abstract Text:

    m hosoyaM Hosoya,j miyazakiJ Miyazaki,t hirabayashiT Hirabayashi,

    In the embryonic smooth muscle of chicken gizzards we found 4 high-Mr-type and 5 low-Mr-type tropomyosin isoforms in addition to alpha- and beta-isoforms reported already. The criteria by which they were identified as tropomyosin isoforms were as follows: 1) anomalous reduction of electrophoretic mobility in the presence of urea, 2) cross reactivity with antisera against tropomyosins, 3) inclusion in a tropomyosin preparation obtained by the usual method for tropomyosin purification, and 4) binding ability to skeletal muscle actin. At the early stages of development, the amounts of these isoforms were larger than those of alpha- and beta-isoforms, but they gradually decreased at later stages and finally disappeared completely after hatching. Our previous study of gizzard smooth muscle showed that the amount ratio of accumulated tropomyosin to gamma-actin was reasonably constant in the development after hatching, while, at the earlier embryonic stages (7-14 d of incubation), it was lower than expected. The isoforms found in this study were present in amounts large enough to bring the ratio at the earlier stages up to the constant amount ratio observed after hatching. Therefore, the coordinate accumulation of actin and tropomyosin was suggested to occur even at the embryonic stages.

    Tropomyosin isoforms in developing chicken gizzard smooth muscle. Publishing Authors By Initials

    m hosoyaM Hosoya,j miyazakiJ Miyazaki,t hirabayashiT Hirabayashi,

    For similar macromolecular substances: polymers: biopolymers: microfilament proteins: tropomyosin research abstracts see: macromolecular substances: polymers: biopolymers: microfilament proteins: tropomyosin research

    PUBMED ID PMID:

    MEDLINE DATE:

    Tropomyosin isoforms in developing chicken gizzard smooth muscle. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Journal of biochemistry

    VOLUME: 105

    Page Numbers: 712-7

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: May

    YEAR: 1989

    Tropomyosin isoforms in developing chicken gizzard smooth muscle. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    Tropomyosin isoforms in developing chicken gizzard smooth muscle. Keywords Mesh Terms:

    KEYWORDS: Tropomyosin

    MESH TERMS: immunology

    Chemical & Substance for Abstract: Tropomyosin isoforms in developing chicken gizzard smooth muscle. Information

    Substance Name: Tropomyosin

    Registry Number: 0

    Grant and Affiliation Information for Tropomyosin isoforms in developing chicken gizzard smooth muscle.

    AFFILIATION: Institute of Biological Sciences, University of Tsukuba, Ibaraki.

    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    MEDLINETA: J Biochem

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