tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation.

tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation. Research Abstract Details 

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tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation. Abstract Text:

Matthew T Palmer,Richard Kirkman,Barry R Kosloff,Peter G Eipers,Casey D Morrow,

An essential step in the replication of all retroviruses is the capture of a cellular tRNA that is used as the primer for reverse transcription. The 3'-terminal 18 nucleotides of the tRNA are complementary to the primer binding site (PBS). Moloney murine leukemia virus (MuLV) preferentially captures tRNA(Pro). To investigate the specificity of primer selection, the PBS of MuLV was altered to be complementary to different tRNAs. Analysis of the infectivity of the virus and stability of the PBS following in vitro replication revealed that MuLV prefers to select tRNA(Pro), tRNA(Gly), or tRNA(Arg). Previous studies from our laboratory have suggested that tRNA primer capture is coordinated with translation. Coincidentally, a cluster of proline, arginine, and glycine precedes the Gag-Pol junction of MuLV. Human immunodeficiency virus type 1 (HIV-1), which prefers tRNA(3)(Lys) as the primer, can be forced to utilize tRNA(Met), tRNA(1,2)(Lys), tRNA(His), or tRNA(Glu), although these viruses replicate poorly. Codons for methionine, lysine, histidine, or glutamic acid are found prior to the Gag-Pol frameshift site. HIV-1 was mutated so that the 5 lysine codons prior to the Gag-Pol frameshift region were specific for tRNA(1,2)(Lys). HIV-1 forced to use tRNA(1,2)(Lys) as the primer, with the mutation of codons specific for tRNA(1,2)(Lys) prior to the Gag-Pol junction, had enhanced infectivity and replicated similarly to the wild-type virus. The results demonstrate that codon preference prior to the Gag-Pol junction influences primer selection and suggest a coordination of Gag-Pol synthesis and acquisition of the tRNA primer required for retrovirus replication.

tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation. Publishing Authors By Initials

MT Palmer,R Kirkman,BR Kosloff,PG Eipers,CD Morrow,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

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tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 4397-404

Journal Abbreviation:

ISSN: 0022-538X

DAY: 14

MONTH: 02

YEAR: 2007

tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: physiology

Chemical & Substance for Abstract: tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation. Information

Substance Name: RNA, Transfer

Registry Number: 9014-25-9

Grant and Affiliation Information for tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation.

AFFILIATION: Department of Cell Biology, University of Alabama at Birmingham, 720 20th Street South, Kaul 802, Birmingham, AL 35294-0024, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI 34749

ACRONYM: AI

MEDLINETA: J Virol

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