TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions.

TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions. Research Abstract Details 

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TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions. Abstract Text:

Brian A Turner,Janet Sparrow,Bolin Cai,Julie Monroe,Takashi Mikawa,Barbara L Hempstead,

Neurotrophins, via activation of Trk receptor tyrosine kinases, serve as mitogens, survival factors and regulators of arborization during retinal development. Brain-derived neurotrophic factor (BDNF) and TrkB regulate neuronal arborization and survival in late retinal development. However, TrkB is expressed during early retinal development where its functions are unclear. To assess TrkB/BDNF actions in the early chick retina, replication-incompetent retroviruses were utilized to over-express a dominant negative truncated form of TrkB (trunc TrkB), or BDNF and effects were assessed at E15. Clones expressing trunc TrkB were smaller than controls, and proliferation and apoptosis assays suggest that decreased clone size correlated with increased cell death when BDNF/TrkB signaling was impaired. Analysis of clonal composition revealed that trunc TrkB over-expression decreased photoreceptor numbers (41%) and increased cell numbers in the middle third of the inner nuclear layer (INL) (23%). Conversely, BDNF over-expression increased photoreceptor numbers (25%) and decreased INL numbers (17%). Photoreceptors over-expressing trunc TrkB demonstrated no increase in apoptosis nor abnormalities in lamination suggesting that TrkB activation is not required for photoreceptor cell survival or migration. These studies suggest that TrkB signaling regulates commitment to and/or differentiation of photoreceptor cells from retinal progenitor cells, identifying a novel role for TrkB/BDNF in regulating cell fate decisions.

TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions. Publishing Authors By Initials

BA Turner,J Sparrow,B Cai,J Monroe,T Mikawa,BL Hempstead,

For similar cells: stem cells research abstracts see: cells: stem cells research

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MEDLINE DATE:

TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Developmental biology

VOLUME: 299

Page Numbers: 455-65

Journal Abbreviation: Dev. Biol.

ISSN: 0012-1606

DAY: 14

MONTH: 08

YEAR: 2006

TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372762

TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions. Keywords Mesh Terms:

KEYWORDS: Stem Cells

MESH TERMS: physiology

Chemical & Substance for Abstract: TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions. Information

Substance Name: Receptor, trkB

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions.

AFFILIATION: Department of Medicine, Weill Medical College of Cornell University, 1300 York Avenue, Room C606, New York, NY 10021, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS30687

ACRONYM: NS

MEDLINETA: Dev Biol

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