Trimethylamine N-oxide stabilizes RNA tertiary structure and attenuates the denaturating effects of urea.

Trimethylamine N-oxide stabilizes RNA tertiary structure and attenuates the denaturating effects of urea. Research Abstract Details 

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Trimethylamine N-oxide stabilizes RNA tertiary structure and attenuates the denaturating effects of urea. Abstract Text:

Thomas C Gluick,Sushma Yadav,

Trimethylamine N-oxide (TMAO) and urea are osmolytes. Osmolytes allow cells to remain viable in harsh or extreme environments. Both TMAO and urea are found in shark and rays at approximate molar ratios of 1:2, respectively. At this ratio TMAO nearly completely counteracts the destabilizing effects that urea has on proteins. We ask whether RNA, which is denatured by urea, is stabilized by TMAO in a manner similar to that seen for proteins. We found that TMAO stabilizes Escherichia coli tRNAfmet tertiary structure and counteracts the denaturing effects of urea at the same ratios found for proteins. Cation binding usually drives RNA tertiary structure formation. These results suggest that tertiary structure stability is not only sensitive to cations but also to the aqueous composition and properties of the solvent. We propose that tertiary structure folding is driven by unfavorable interactions between TMAO and the phosphodiester backbone.

Trimethylamine N-oxide stabilizes RNA tertiary structure and attenuates the denaturating effects of urea. Publishing Authors By Initials

TC Gluick,S Yadav,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Trimethylamine N-oxide stabilizes RNA tertiary structure and attenuates the denaturating effects of urea. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 4418-9

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 16

MONTH: Apr

YEAR: 2003

Trimethylamine N-oxide stabilizes RNA tertiary structure and attenuates the denaturating effects of urea. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Trimethylamine N-oxide stabilizes RNA tertiary structure and attenuates the denaturating effects of urea. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: chemistry

Chemical & Substance for Abstract: Trimethylamine N-oxide stabilizes RNA tertiary structure and attenuates the denaturating effects of urea. Information

Substance Name: Urea

Registry Number: 57-13-6

Grant and Affiliation Information for Trimethylamine N-oxide stabilizes RNA tertiary structure and attenuates the denaturating effects of urea.

AFFILIATION: Department of Chemistry and Biochemistry, P.O. Box 19065, The University of Texas at Arlington, Arlington, TX 76019, USA. tcgluick@uta.edu

Country: United States

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MEDLINETA: J Am Chem Soc

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