Trf4 is a Useful Gene for Discrimination of Candida tropicalis from other Medically Important Candida Species.

Trf4 is a Useful Gene for Discrimination of Candida tropicalis from other Medically Important Candida Species. Research Abstract Details 

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Trf4 is a Useful Gene for Discrimination of Candida tropicalis from other Medically Important Candida Species. Abstract Text:

Yingqian Kang,Soji Iida,Setsuya Yamamoto,Takahisa Kogure,Reiko Tanaka,Yuzuru Mikami,

Comparative studies of random amplified polymorphic DNA (RAPD) band patterns of Candida tropicalis with those of clinically important Candida species have shown the presence of specific RAPD bands for C. tropicalis. A band specific to C. tropicalis strains (ca. 400 bp) was extracted and sequenced. It was found to belong to a fragment of the Trf4 gene, which is essential for growth of these strains and has a characteristic sequence of C. tropicalis. A PCR primer was designed specifically for C. tropicalis which amplifies the 324 bp band. The PCR primer amplified DNA products for all C. tropicalis strains tested, but did not amplify any PCR bands from C. albicans, C. dubliniensis, C. glabrata, C. guilliermondii, C. kefer, C. krusei, C. parapsilosis, or C. zeylanoides. Usefulness of the PCR primer in differentiating from clinical isolates of other fungal species is discussed.

Trf4 is a Useful Gene for Discrimination of Candida tropicalis from other Medically Important Candida Species. Publishing Authors By Initials

Y Kang,S Iida,S Yamamoto,T Kogure,R Tanaka,Y Mikami,

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Trf4 is a Useful Gene for Discrimination of Candida tropicalis from other Medically Important Candida Species. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nihon Ishinkin Gakkai zasshi = Japanese journal of

VOLUME: 49

Page Numbers: 39-43

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ISSN: 0916-4804

DAY: 15

MONTH: 02

YEAR: 2008

Trf4 is a Useful Gene for Discrimination of Candida tropicalis from other Medically Important Candida Species. Information

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LANGUAGE: eng

NlmUniqueID: 9425640

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Grant and Affiliation Information for Trf4 is a Useful Gene for Discrimination of Candida tropicalis from other Medically Important Candida Species.

AFFILIATION: Medical Mycology Research Center (MMRC) , Chiba University.

Country: Japan

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MEDLINETA: Nippon Ishinkin Gakkai Zasshi

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