Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats.

Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats. Research Abstract Details 

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Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats. Abstract Text:

D Hunter Best,William B Coleman,

BACKGROUND/AIMS: Liver regeneration after partial hepatectomy (PH) in retrorsine-exposed rats is accomplished through proliferation and differentiation of small hepatocyte-like progenitor cells (SHPCs). The cells of origin of SHPCs are not known. We investigated the possibility that SHPCs are directly derived from oval cells, a known liver progenitor cell, by combining the retrorsine/PH (RP) model with 2-acetamidofluorene (2-AAF), an anti-mitotic agent that elicits an oval cell reaction in response to liver deficit. METHODS: Male Fischer 344 rats were treated with retrorsine (30 mg/kg ip) at 6 and 8 weeks of age, with PH 5 weeks after the final treatment. Seven days prior to PH, a 21-day 2-AAF (50mg) time-release pellet was inserted subcutaneously. Livers were harvested at 3, 7, 10, 14, and 21-days post-PH. RESULTS: Liver sections from animals treated with 2-AAF/retrorsine/PH (2-AAF/RP) contain significant numbers of proliferating oval cells, but no SHPCs at 7-days post-PH, while RP animals exhibit significant numbers of SHPCs and minimal oval cell reaction. Between 10 and 14-days post-PH, new hepatocyte clusters appear in 2-AAF/RP treated rats. Labeling of proliferating oval cells with BrdU at 6-days post-PH demonstrated that these new hepatocytes represent the progeny of differentiating oval cells. CONCLUSIONS: The observed differences in progenitor cell responses between 2-AAF/RP and RP animals strongly suggest that SHPCs are not the progeny of oval cell precursors, but represent an independent liver progenitor cell population.

Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats. Publishing Authors By Initials

DH Best,WB Coleman,

For similar cells: stem cells research abstracts see: cells: stem cells research

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Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of hepatology

VOLUME: 46

Page Numbers: 1055-63

Journal Abbreviation:

ISSN: 0168-8278

DAY: 8

MONTH: 03

YEAR: 2007

Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8503886

Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats. Keywords Mesh Terms:

KEYWORDS: Stem Cells

MESH TERMS: metabolism

Chemical & Substance for Abstract: Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats. Information

Substance Name: 2-Acetylaminofluorene

Registry Number: 53-96-3

Grant and Affiliation Information for Treatment with 2-AAF blocks the small hepatocyte-like progenitor cell response in retrorsine-exposed rats.

AFFILIATION: Department of Pathology and Laboratory Medicine, Curriculum in Toxicology, UNC Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.

Country: England

AGENCY: United States NIEHS

GRANT: T32 ES 07017

ACRONYM: ES

MEDLINETA: J Hepatol

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