Treatment of osteonecrosis of the femoral head with hBMP-2-gene-modified tissue-engineered bone in goats.

Treatment of osteonecrosis of the femoral head with hBMP-2-gene-modified tissue-engineered bone in goats. Research Abstract Details 

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Treatment of osteonecrosis of the femoral head with hBMP-2-gene-modified tissue-engineered bone in goats. Abstract Text:

T T Tang,B Lu,B Yue,X H Xie,Y Z Xie,K R Dai,J X Lu,J R Lou,T T Tang,B Lu,B Yue,X H Xie,Y Z Xie,K R Dai,J X Lu,J R Lou,

The efficacy of beta-tricalcium phosphate (beta-TCP) loaded with bone morphogenetic protein-2 (BMP-2)-gene-modified bone-marrow mesenchymal stem cells (BMSCs) was evaluated for the repair of experimentally-induced osteonecrosis of the femoral head in goats. Bilateral early-stage osteonecrosis was induced in adult goats three weeks after ligation of the lateral and medial circumflex arteries and delivery of liquid nitrogen into the femoral head. After core decompression, porous beta-TCP loaded with BMP-2 gene- or beta-galactosidase (gal)-gene-transduced BMSCs was implanted into the left and right femoral heads, respectively. At 16 weeks after implantation, there was collapse of the femoral head in the untreated group but not in the BMP-2 or beta-gal groups. The femoral heads in the BMP-2 group had a normal density and surface, while those in the beta-gal group presented with a low density and an irregular surface. Histologically, new bone and fibrous tissue were formed in the macropores of the beta-TCP. Sixteen weeks after implantation, lamellar bone had formed in the BMP-2 group, but there were some empty cavities and residual fibrous tissue in the beta-gal group. The new bone volume in the BMP-2 group was significantly higher than that in the beta-gal group. The maximum compressive strength and Young's modulus of the repaired tissue in the BMP-2 group were similar to those of normal bone and significantly higher than those in the beta-gal group. Our findings indicate that porous beta-TCP loaded with BMP-2-gene-transduced BMSCs are capable of repairing early-stage, experimentally-induced osteonecrosis of the femoral head and of restoring its mechanical function.

Treatment of osteonecrosis of the femoral head with hBMP-2-gene-modified tissue-engineered bone in goats. Publishing Authors By Initials

TT Tang,B Lu,B Yue,XH Xie,YZ Xie,KR Dai,JX Lu,JR Lou,TT Tang,B Lu,B Yue,XH Xie,YZ Xie,KR Dai,JX Lu,JR Lou,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Treatment of osteonecrosis of the femoral head with hBMP-2-gene-modified tissue-engineered bone in goats. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of bone and joint surgery. British vo

VOLUME: 89

Page Numbers: 127-9

Journal Abbreviation:

ISSN: 0301-620X

DAY: 19

MONTH: Jan

YEAR: 2007

Treatment of osteonecrosis of the femoral head with hBMP-2-gene-modified tissue-engineered bone in goats. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375355

Treatment of osteonecrosis of the femoral head with hBMP-2-gene-modified tissue-engineered bone in goats. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Treatment of osteonecrosis of the femoral head with hBMP-2-gene-modified tissue-engineered bone in goats. Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for Treatment of osteonecrosis of the femoral head with hBMP-2-gene-modified tissue-engineered bone in goats.

AFFILIATION: Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong, University School of Medicine, Shanghai, the People's [corrected] Republic of China. tingtingtang@hotmail.com

Country: England

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MEDLINETA: J Bone Joint Surg Br

REFSOURCE: J Bone Joint Surg Br. 2007 Mar;89(3):421

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