Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes.

Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes. Research Abstract Details 

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Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes. Abstract Text:

Ludivine Sinzelle,Vladimir V Kapitonov,Dawid P Grzela,Tobias Jursch,Jerzy Jurka,Zsuzsanna , Ivics,Ludivine Sinzelle,Vladimir V Kapitonov,Dawid P Grzela,Tobias Jursch,Jerzy Jurka,Zsuzsanna , Ivics,

Ancient, inactive copies of transposable elements of the PIF/Harbinger superfamily have been described in vertebrates. We reconstructed components of the Harbinger3_DR transposon in zebrafish, including a transposase and a second, transposon-encoded protein that has a Myb-like trihelix domain. The reconstructed Harbinger transposon shows efficient cut-and-paste transposition in human cells and preferentially inserts into a 15-bp consensus target sequence. The Myb-like protein is required for transposition and physically interacts with the N-terminal region of the transposase via its C-terminal domain. The Myb-like protein enables transposition in part by promoting nuclear import of the transposase, by directly binding to subterminal regions of the transposon, and by recruiting the transposase to the transposon ends. We investigated the functions of two transposon-derived human proteins: HARBI1, a domesticated transposase-derived protein, and NAIF1, which contains a trihelix motif similar to that described in the Myb-like protein. Physical interaction, subcellular localization, and DNA-binding activities of HARBI1 and NAIF1 suggest strong functional homologies between the Harbinger3_DR system and their related, host-encoded counterparts. The Harbinger transposon will serve as a useful experimental system for transposon biology and for investigating the enzymatic functions of domesticated, transposon-derived cellular genes.

Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes. Publishing Authors By Initials

L Sinzelle,VV Kapitonov,DP Grzela,T Jursch,J Jurka,Z ,Z Ivics,L Sinzelle,VV Kapitonov,DP Grzela,T Jursch,J Jurka,Z ,Z Ivics,

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Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 105

Page Numbers: 4715-20

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 1091-6490

DAY: 13

MONTH: 03

YEAR: 2008

Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

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Grant and Affiliation Information for Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes.

AFFILIATION: Max Delbrück Center for Molecular Medicine, 13092 Berlin, Germany.

Country: United States

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MEDLINETA: Proc Natl Acad Sci U S A

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