Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression.

Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression. Research Abstract Details 

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Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression. Abstract Text:

Kyung-Yeon Han,Hyuk-Seong Seo,Jong-Am Song,Keum-Young Ahn,Jin-Seung Park,Jeewon Lee,Kyung-Yeon Han,Hyuk-Seong Seo,Jong-Am Song,Keum-Young Ahn,Jin-Seung Park,Jeewon Lee,

The Escherichia coli proteome response to the stressor GdnHCl was analyzed through 2-dimensional gel electrophoresis (2-DE). We identified PotD (spermidine/putrescine-binding periplasmic protein) and Crr [glucose-specific phosphotransferase (PTS) enzyme IIA component] as a stress-responsive protein. Even under a stress situation where the total number of soluble proteins decreased by about 10%, 3.5- and 2.2-fold increase was observed in the synthesis of PotD and Crr, respectively. As fusion partners, PotD and Crr dramatically increased the solubility of many aggregation-prone heterologous proteins [e.g. human minipro-insulin (mp-INS), human epidermal growth factor (EGF), human prepro-ghrelin (ppGRN), human interleukin-2(hIL-2), human activation induced cytidine deaminase (AID), human glutamate decarboxylase (GAD(448-585)), Pseudomonas putida cutinase (CUT), human ferritin light chain (hFTN-L), human granulocyte colony-stimulating factor (G-CSF), and cold autoinflammatory syndrome1 protein (NALP3) Nacht domain (NACHT)] in the E. coli cytoplasm. Presumably PotD and Crr were very effective in shielding interactive surfaces of heterologous proteins associated with non-specific protein-protein interactions leading to the formation of inclusion bodies most likely due to intrinsic high folding efficiency, chaperone-like activity, or a combination of both factors. Both the stress-induced proteins were well suited for the production of a biologically active fusion mutant of P. putida cutinase that can be expected to be of biotechnological and commercial interest.

Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression. Publishing Authors By Initials

KY Han,HS Seo,JA Song,KY Ahn,JS Park,J Lee,KY Han,HS Seo,JA Song,KY Ahn,JS Park,J Lee,

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Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochimica et biophysica acta

VOLUME: 1774

Page Numbers: 1536-43

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 4

MONTH: 10

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression.

AFFILIATION: Department of Chemical and Biological Engineering, Korea University, Anam-Dong 5-1, Sungbuk-Ku, Seoul 136-713, South Korea.

Country: Netherlands

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MEDLINETA: Biochim Biophys Acta

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