Transgenic expression of Helicobacter pylori CagA induces gastrointestinal and hematopoietic neoplasms in mouse.

Transgenic expression of Helicobacter pylori CagA induces gastrointestinal and hematopoietic neoplasms in mouse. Research Abstract Details 

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Transgenic expression of Helicobacter pylori CagA induces gastrointestinal and hematopoietic neoplasms in mouse. Abstract Text:

Naomi Ohnishi,Hitomi Yuasa,Shinya Tanaka,Hirofumi Sawa,Motohiro Miura,Atsushi Matsui,Hideaki Higashi,Manabu Musashi,Kazuya Iwabuchi,Misao Suzuki,Gen Yamada,Takeshi Azuma,Masanori Hatakeyama,Naomi Ohnishi,Hitomi Yuasa,Shinya Tanaka,Hirofumi Sawa,Motohiro Miura,Atsushi Matsui,Hideaki Higashi,Manabu Musashi,Kazuya Iwabuchi,Misao Suzuki,Gen Yamada,Takeshi Azuma,Masanori Hatakeyama,Naomi Ohnishi,Hitomi Yuasa,Shinya Tanaka,Hirofumi Sawa,Motohiro Miura,Atsushi Matsui,Hideaki Higashi,Manabu Musashi,Kazuya Iwabuchi,Misao Suzuki,Gen Yamada,Takeshi Azuma,Masanori Hatakeyama,

Infection with cagA-positive Helicobacter pylori is associated with gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue (MALT) lymphoma of B cell origin. The cagA-encoded CagA protein is delivered into gastric epithelial cells via the bacterial type IV secretion system and, upon tyrosine phosphorylation by Src family kinases, specifically binds to and aberrantly activates SHP-2 tyrosine phosphatase, a bona fide oncoprotein in human malignancies. CagA also elicits junctional and polarity defects in epithelial cells by interacting with and inhibiting partitioning-defective 1 (PAR1)/microtubule affinity-regulating kinase (MARK) independently of CagA tyrosine phosphorylation. Despite these CagA activities that contribute to neoplastic transformation, a causal link between CagA and in vivo oncogenesis remains unknown. Here, we generated transgenic mice expressing wild-type or phosphorylation-resistant CagA throughout the body or predominantly in the stomach. Wild-type CagA transgenic mice showed gastric epithelial hyperplasia and some of the mice developed gastric polyps and adenocarcinomas of the stomach and small intestine. Systemic expression of wild-type CagA further induced leukocytosis with IL-3/GM-CSF hypersensitivity and some mice developed myeloid leukemias and B cell lymphomas, the hematological malignancies also caused by gain-of-function SHP-2 mutations. Such pathological abnormalities were not observed in transgenic mice expressing phosphorylation-resistant CagA. These results provide first direct evidence for the role of CagA as a bacterium-derived oncoprotein (bacterial oncoprotein) that acts in mammals and further indicate the importance of CagA tyrosine phosphorylation, which enables CagA to deregulate SHP-2, in the development of H. pylori-associated neoplasms.

Transgenic expression of Helicobacter pylori CagA induces gastrointestinal and hematopoietic neoplasms in mouse. Publishing Authors By Initials

N Ohnishi,H Yuasa,S Tanaka,H Sawa,M Miura,A Matsui,H Higashi,M Musashi,K Iwabuchi,M Suzuki,G Yamada,T Azuma,M Hatakeyama,N Ohnishi,H Yuasa,S Tanaka,H Sawa,M Miura,A Matsui,H Higashi,M Musashi,K Iwabuchi,M Suzuki,G Yamada,T Azuma,M Hatakeyama,N Ohnishi,H Yuasa,S Tanaka,H Sawa,M Miura,A Matsui,H Higashi,M Musashi,K Iwabuchi,M Suzuki,G Yamada,T Azuma,M Hatakeyama,

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Transgenic expression of Helicobacter pylori CagA induces gastrointestinal and hematopoietic neoplasms in mouse. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 105

Page Numbers: 1003-8

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 1091-6490

DAY: 11

MONTH: 01

YEAR: 2008

Transgenic expression of Helicobacter pylori CagA induces gastrointestinal and hematopoietic neoplasms in mouse. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

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AFFILIATION: Division of Molecular Oncology, Institute for Genetic Medicine and Division of Chemistry, Graduate School of Science, Health Administration Center, and Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan

Country: United States

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MEDLINETA: Proc Natl Acad Sci U S A

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